Scherer P E, Bickel P E, Kotler M, Lodish H F
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Nat Biotechnol. 1998 Jun;16(6):581-6. doi: 10.1038/nbt0698-581.
To identify and clone genes that encode cell- or tissue-specific secreted and surface proteins, a polyclonal antiserum was raised against a complex mixture of surface or secreted proteins from the target cell, followed by immunodepletion of antibodies that recognize proteins from a nontarget cell or tissue. The depleted antiserum is used to screen bacteriophage cDNA expression libraries. Because of our interest in how adipocytes communicate with other cells, we have used this method to clone cDNAs encoding secreted and plasma membrane proteins that are induced during adipocyte differentiation. We describe several of these, including a novel plasma membrane-associated protein, S3-12.
为了鉴定和克隆编码细胞或组织特异性分泌蛋白和表面蛋白的基因，制备了针对靶细胞表面或分泌蛋白复杂混合物的多克隆抗血清，随后免疫去除识别非靶细胞或组织蛋白的抗体。用去除后的抗血清筛选噬菌体cDNA表达文库。由于我们对脂肪细胞如何与其他细胞通讯感兴趣，我们已使用此方法克隆在脂肪细胞分化过程中诱导表达的分泌蛋白和质膜蛋白的cDNA。我们描述了其中几种，包括一种新型质膜相关蛋白S3-12。
