Zhang Qiaochu, Zhang Pengpeng, Li Bingcheng, Dang Hongwei, Jiang Jinfang, Meng Lian, Zhang Haijun, Zhang Yangyang, Wang Xiaomeng, Li Qianru, Wang Yang, Liu Chunxia, Li Feng
Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi 832002, China.
Department of Pathology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Diseases, Beijing, 100032 China.
J Cancer. 2020 Apr 7;11(14):4081-4090. doi: 10.7150/jca.41736. eCollection 2020.
: Liposarcoma is a mesenchymal malignant tumor characterized by adipocyte differentiation which is divided into four subtypes with different prognosis. Accurate histopathological diagnosis is essential for precise treatment. Perilipins, including PLIN1, PLIN2, PLIN3, PLIN4, PLIN5, is a family of lipid droplet-associated proteins that participate in lipid metabolism regulation. The role that perilipins play in sarcomas is not clear. This study aims to assess perilipins expression in subtypes of liposarcoma and various non-lipomatous sarcomas. : A large set of 245 soft tissue sarcoma paraffin-embedded samples including 66 liposarcomas and 179 non-lipomatous sarcomas were collected for tissue microarray and immunohistochemistry to assess perilipins expression. : PLIN1 expression was shown in most liposarcomas (41/66) and was absent in non-lipomatous sarcomas (0/179). PLIN4 expression was shown in some liposarcomas (21/66) and was almost negative in non-lipomatous sarcomas (2/179). PLIN1 and PLIN4 expressions in liposarcoma were higher (both P<0.001) than those in non-lipomatous sarcoma. Both PLIN1 and PLIN4 also had a significant difference in liposarcoma subtypes (both P<0.001). PLIN2, PLIN3 and PLIN5 were widely expressed in liposarcomas, rhabdomyosarcomas, leiomyosarcomas, dermatofibrosarcoma protuberans, undifferentiated sarcomas, fibrosarcomas, Ewing's sarcomas and epithelioid sarcomas. PLIN2, PLIN3 and PLIN5 expressions were significantly different among non-lipomatous sarcoma (all P<0.01). Except for PLIN3, the expression of the other four perilipin members in liposarcoma was pairwise related. : PLIN1 and PLIN4 can be used as diagnostic markers of liposarcoma and to differentiate liposarcoma subtypes. The combined application of whole perilipin family immunohistochemistry may help to distinguish differently differentiated sarcomas.
脂肪肉瘤是一种以脂肪细胞分化为特征的间叶性恶性肿瘤，分为四种预后不同的亚型。准确的组织病理学诊断对于精确治疗至关重要。脂联素包括PLIN1、PLIN2、PLIN3、PLIN4、PLIN5，是一族与脂滴相关的蛋白质，参与脂质代谢调节。脂联素在肉瘤中的作用尚不清楚。本研究旨在评估脂联素在脂肪肉瘤各亚型及各种非脂肪瘤性肉瘤中的表达情况。
收集了包括66例脂肪肉瘤和179例非脂肪瘤性肉瘤在内的245例软组织肉瘤石蜡包埋样本，制作组织芯片并进行免疫组织化学检测以评估脂联素的表达情况。
大多数脂肪肉瘤（41/66）中可见PLIN1表达，非脂肪瘤性肉瘤中无PLIN1表达（0/179）。部分脂肪肉瘤（21/66）中可见PLIN4表达，非脂肪瘤性肉瘤中PLIN4表达几乎为阴性（2/179）。脂肪肉瘤中PLIN1和PLIN4的表达高于非脂肪瘤性肉瘤（均P<0.001）。PLIN1和PLIN4在脂肪肉瘤各亚型中也存在显著差异（均P<0.001）。PLIN2、PLIN3和PLIN5在脂肪肉瘤、横纹肌肉瘤、平滑肌肉瘤、隆突性皮肤纤维肉瘤、未分化肉瘤、纤维肉瘤、尤因肉瘤和上皮样肉瘤中广泛表达。PLIN2、PLIN3和PLIN5在非脂肪瘤性肉瘤中的表达存在显著差异（均P<0.01）。除PLIN3外，脂肪肉瘤中其他四种脂联素成员的表达两两相关。
PLIN1和PLIN4可作为脂肪肉瘤的诊断标志物及鉴别脂肪肉瘤亚型的指标。脂联素全家族免疫组织化学联合应用可能有助于鉴别不同分化程度的肉瘤。
