Frew M E, Donaldson K
Haematology Department, Fife Area Laboratory, Scotland, UK.
Br J Biomed Sci. 1997 Dec;54(4):244-50.
It is suggested that monocytes in patients with chronic myelomonocytic leukaemia (CMML) or chronic myeloid leukaemia (CML) with monocytosis have morphological/functional abnormalities which cause inaccurate counting in automated analysers. In this study, monocytes in 21 normal and 14 CMML blood samples were subjected to morphological analysis and were counted by the manual reference method, three automated analysers and esterase staining. Morphological analysis showed no significant difference between control and CMML monocytes. The alpha-naphthyl acetate esterase scores, a measure of monocyte function, showed a reduction of 40% in CMML monocytes compared to controls. Counts by analysers showed that the Sysmex NE 8000 was the least accurate for CMML monocyte counts and that the Coulter STK-S and Sysmex SE 9000 gave results closer to the manually counted standards.
有人提出，慢性粒单核细胞白血病（CMML）患者或伴有单核细胞增多的慢性髓性白血病（CML）患者的单核细胞存在形态学/功能异常，这会导致自动分析仪计数不准确。在本研究中，对21份正常血液样本和14份CMML血液样本中的单核细胞进行形态学分析，并通过手工参考方法、三种自动分析仪和酯酶染色进行计数。形态学分析显示，对照组和CMML单核细胞之间无显著差异。作为单核细胞功能指标的α-萘乙酸酯酶评分显示，与对照组相比，CMML单核细胞减少了40%。分析仪计数结果显示，Sysmex NE 8000对CMML单核细胞计数的准确性最低，而Coulter STK-S和Sysmex SE 9000给出的结果更接近手工计数标准。
