Jahrling P B, Navarro E, Scherer W F
Arch Virol. 1976;51(1-2):23-35. doi: 10.1007/BF01317831.
Venezuelan encephalitis (VEE) virus strains, which differ in virulence for adult hamsters, were compared with respect to (a) sensitivity to hamster interferon (IF) in vitro and in vivo and (b) induction of IF in plasma and target tissues (spleen, bone marrow and brain) following subcutaneous inoculation. In vitro, in cultures of a continuous line of hamster kidney cells, hamster interferon inhibited the replication of a benign VEE strain (BeAr 35,645) more than another benign strain (TC-83 vaccine) or two hamster-virulent strains (68 U 201 and Trinidad donkey). In vivo, in hamsters given poly I: poly C 24 hours before virus to induce interferon formation, BeAr 35,645 and TC-83 virus infections were prevented more frequently than infections with virulent strains Trinidad donkey and 68U201. Benign VEE strains BeAr 35,645 and TC-83 induced only slightly lower concentrations of IF in plasma, bone marrow, spleen and brain than virulent strains Trinidad donkey, 63Z21 and 68U201. However, concentrations of infectious BeAr 35,645 virus were significantly lower in these tissues than virulent strains, resulting in higher ratios of IF: invectious virus, suggesting efficient interferon induction. Benign strain TC-83 showed irregular relationships between IF and infectious virus in plasma or blood and tissues, Splenectomy significantly depressed plasma plasma IF responses to TC-83 virus 20 to 30 hours after inoculation. Interferon appears to be a factor that influences virulence of VEE viruses for hamsters.
委内瑞拉脑炎（VEE）病毒株对成年仓鼠的毒力不同，就以下方面进行了比较：（a）在体外和体内对仓鼠干扰素（IF）的敏感性，以及（b）皮下接种后血浆和靶组织（脾脏、骨髓和脑）中IF的诱导情况。在体外，在仓鼠肾细胞连续系培养物中，仓鼠干扰素对良性VEE病毒株（BeAr 35,645）复制的抑制作用比对另一良性株（TC - 83疫苗）或两种仓鼠强毒株（68 U 201和特立尼达驴毒株）更强。在体内，在病毒接种前24小时给予聚肌苷酸:聚胞苷酸（poly I: poly C）以诱导干扰素形成的仓鼠中，BeAr 35,645和TC - 83病毒感染的预防频率比强毒株特立尼达驴和68U201感染更高。良性VEE病毒株BeAr 35,645和TC - 83在血浆、骨髓、脾脏和脑中诱导的IF浓度仅比特立尼达驴、63Z21和68U201等强毒株略低。然而，这些组织中传染性BeAr 35,645病毒的浓度明显低于强毒株，导致IF:传染性病毒的比率更高，表明干扰素诱导效率高。良性毒株TC - 83在血浆或血液及组织中IF与传染性病毒之间呈现不规则关系。脾切除显著降低了接种后20至30小时血浆中对TC - 83病毒的IF反应。干扰素似乎是影响VEE病毒对仓鼠毒力的一个因素。
