McDonald P P, Gasperini S, Calzetti F, Cassatella M A
Institute of General Pathology, Verona, Italy.
Cell Immunol. 1998 Feb 25;184(1):45-50. doi: 10.1006/cimm.1998.1255.
In this report, we show that interferon-gamma (IFN-gamma) modulates the production of IL-1ra in activated human neutrophils. In lipopolysaccharide-stimulated cells, IFN-gamma increased the release of IL-1ra without modulating IL-1ra mRNA accumulation; under these conditions, IFN-gamma only marginally enhanced IL-1ra de novo synthesis, while IL-1ra was more efficiently secreted. In response to the formylated peptide, fMLP, neutrophils released small but significant amounts of IL-1ra, yet without an increase in IL-1ra mRNA over constitutive levels. Following IFN-gamma treatment, however, the fMLP-elicited IL-1ra production was greatly potentiated, and this was accompanied by a transient increased accumulation of IL-1ra mRNA. Finally, opsonized yeast particles were found to induce IL-1ra formation at late incubation times, and prior treatment of neutrophils with IFN-gamma moderately enhanced this response. Collectively, our results demonstrate that in neutrophils activated by different classes of agonists, IFN-gamma modulates the release of IL-1ra by acting through distinct mechanisms.
在本报告中，我们表明干扰素-γ（IFN-γ）可调节活化的人中性粒细胞中白细胞介素-1受体拮抗剂（IL-1ra）的产生。在脂多糖刺激的细胞中，IFN-γ增加了IL-1ra的释放，但未调节IL-1ra mRNA的积累；在这些条件下，IFN-γ仅略微增强了IL-1ra的从头合成，而IL-1ra的分泌更有效。响应于甲酰化肽fMLP，中性粒细胞释放少量但显著量的IL-1ra，但IL-1ra mRNA水平并未超过组成水平而增加。然而，在IFN-γ处理后，fMLP诱导的IL-1ra产生大大增强，并且这伴随着IL-1ra mRNA的瞬时积累增加。最后，发现调理过的酵母颗粒在孵育后期诱导IL-1ra形成，并且用IFN-γ预先处理中性粒细胞适度增强了这种反应。总体而言，我们的结果表明，在由不同类别的激动剂激活的中性粒细胞中，IFN-γ通过不同机制调节IL-1ra的释放。
