Mojzis J, Nicák A, Guzy J, Kron I, Mirossay L
Department of Pharmacology, Medical Faculty, P.J. Safarik University Kosice, Slovak Republic.
Free Radic Biol Med. 1998 May;24(7-8):1347-51. doi: 10.1016/s0891-5849(97)00462-0.
Our previous study showed that stobadine is effective against ischemia/reperfusion-induced gastric mucosal injury. The present study examined the ability of stobadine to protect erythrocyte membrane against free radical injury after long-term carbon tetrachloride (CCl4) application. The erythrocyte membrane changes were established using colloid-osmotic hemolysis. The significant increase of colloid-osmotic hemolysis was found in animals treated with CCl4. CCl4 also increased formation of thiobarbituric acid-reactive substances (TBARs) and decreased thiol group content. Stobadine in both doses (10.0 and 20.0 mg.kg-1) protected erythrocyte membrane against CCl4-induced injury. The membrane lipid bilayer is the most affected part of the erythrocyte membrane. In presence of stobadine, CCl4-induced lipid peroxidation was partially or totally prevented whereas the level of total membrane thiols was increased. Based on these results, it can be concluded that protective effect of stobadine on CCl4-induced erythrocyte membrane changes should be related to its antioxidant properties.
我们之前的研究表明，司他定对缺血/再灌注诱导的胃黏膜损伤有效。本研究检测了长期应用四氯化碳（CCl4）后司他定保护红细胞膜免受自由基损伤的能力。利用胶体渗透压溶血法确定红细胞膜的变化。在接受CCl4治疗的动物中发现胶体渗透压溶血显著增加。CCl4还增加了硫代巴比妥酸反应性物质（TBARs）的形成并降低了巯基含量。两种剂量（10.0和20.0 mg·kg-1）的司他定都能保护红细胞膜免受CCl4诱导的损伤。膜脂双层是红细胞膜受影响最大的部分。在司他定存在的情况下，CCl4诱导的脂质过氧化被部分或完全阻止，而总膜巯基水平增加。基于这些结果，可以得出结论，司他定对CCl4诱导的红细胞膜变化的保护作用应与其抗氧化特性有关。
