Pomfy M, Nicák A, Mojzis J, Húska J, Benes L
Department of Histology and Embryology, Medical Faculty University P.J. Safarik, Kosice, Slovak Republic.
Mol Chem Neuropathol. 1995 Jun-Aug;25(2-3):115-22. doi: 10.1007/BF02960906.
Serious brain ischemia was induced by occlusion of cerebral arteries in dogs. The occlusion time was 7 min. The blood was collected at various intervals of reperfusion (5, 60, 180, 240 min and 24 h). Thirty minutes before ischemization, stobadine was given (1, 2, or 5 mg/kg). The changes of erythrocyte membrane fluidity were evaluated using colloid-osmotic hemolysis induced by brilliant cresyl blue. In the control group (without stobadine) the colloid-osmotic hemolysis was significantly increased immediately after ischemization and after 5 and 60 min. However, after 240 min of reperfusion, a significant decrease of hemolysis was observed. The increase of colloid-osmotic hemolysis after ischemization in the control group was prevented after stobadine pretreatment. The thrombotization of microcirculation that was observed in the control group was not present after stobadine pretreatment.
通过闭塞犬脑动脉诱导严重脑缺血。闭塞时间为7分钟。在再灌注的不同时间间隔（5、60、180、240分钟和24小时）采集血液。在缺血前30分钟给予司托巴定（1、2或5毫克/千克）。使用灿烂甲酚蓝诱导的胶体渗透溶血评估红细胞膜流动性的变化。在对照组（未用司托巴定）中，缺血后以及5分钟和60分钟后胶体渗透溶血显著增加。然而，再灌注240分钟后，观察到溶血显著降低。司托巴定预处理后可防止对照组缺血后胶体渗透溶血的增加。司托巴定预处理后未观察到对照组中出现的微循环血栓形成。
