Saeed M, van Dijke C F, Mann J S, Wendland M F, Rosenau W, Higgins C B, Brasch R C
Department of Radiology, University of California, San Francisco 94143, USA.
J Magn Reson Imaging. 1998 May-Jun;8(3):561-7. doi: 10.1002/jmri.1880080308.
A macromolecular MR contrast medium (MMCM) designed to permit histochemical staining and specific tissue localization, albumin-(biotin)10-(Gd-DTPA)25 (Bio-Alb-Gd), was used in a rat model of reperfused myocardial infarction to confirm the presence and distribution of microvascular hyperpermeability. T1-weighted spin-echo images were acquired before and after administration of Bio-Alb-Gd. An avidin-biotin-complex (ABC) stain, specific for the biotinylated MR contrast medium, was used to define the MMCM distribution and to detect any regional change in microvascular permeability related to infarction. Immediately after Bio-Alb-Gd administration, the infarcted region was enhanced, with greatest signal intensity noted at the rim and less at the center. There was a gradual increase in signal intensity of the initially hypointense central region. The steady increase in signal intensity of the central region suggested convection transport of MMCM through the interstitial space and its influx into cellular compartment after leakage from the vascular compartment. Histologic findings confirmed regional microvascular hyperpermeability corresponding to the site of infarction and a predominant rim distribution of the MMCM. Bio-Alb-Gd was identified at high microscopic power in the intravascular, interstitial, and intracellular spaces at the periphery of reperfused infarcted myocardium. Bio-Alb-Gd can be used as an MR contrast medium in reperfused infarcted myocardium to confirm the existence and to localize altered microvascular permeability to macromolecules. Bio-Alb-Gd contrast technique removes all the ambiguity between the distribution of the MR or other imaging contrast agent and the distribution of the substrate for histochemical staining.
一种旨在实现组织化学染色和特定组织定位的大分子磁共振造影剂（MMCM），即白蛋白-（生物素）10-（钆-二乙三胺五乙酸）25（Bio-Alb-Gd），被用于再灌注心肌梗死大鼠模型，以确认微血管高通透性的存在和分布。在注射Bio-Alb-Gd前后采集T1加权自旋回波图像。使用对生物素化磁共振造影剂特异的抗生物素蛋白-生物素复合物（ABC）染色来确定MMCM的分布，并检测与梗死相关的微血管通透性的任何区域变化。注射Bio-Alb-Gd后立即观察到梗死区域强化，边缘信号强度最高，中心较低。最初低信号的中心区域信号强度逐渐增加。中心区域信号强度的持续增加表明MMCM通过间质空间的对流运输及其从血管腔渗漏后流入细胞腔。组织学结果证实梗死部位存在区域微血管高通透性，且MMCM主要分布在边缘。在再灌注梗死心肌周边的血管内、间质和细胞内空间，在高倍显微镜下可识别出Bio-Alb-Gd。Bio-Alb-Gd可作为再灌注梗死心肌的磁共振造影剂，以确认大分子微血管通透性改变的存在并对其进行定位。Bio-Alb-Gd造影技术消除了磁共振或其他成像造影剂的分布与组织化学染色底物分布之间的所有模糊性。
