Torres M A, Onouchi H, Hamada S, Machida C, Hammond-Kosack K E, Jones J D
Sainsbury Laboratory, John Innes Centre, Norwich, UK.
Plant J. 1998 May;14(3):365-70. doi: 10.1046/j.1365-313x.1998.00136.x.
An NADPH oxidase analogous to that in mammalian phagocytes has been hypothesized to produce reactive oxygen species (ROS) in the plant defence response. A. thaliana contains at least six gp91phox homologues, designated AtrbohA-F (A. thaliana Respiratory Burst Oxidase Homologues), which map to different positions. Transcripts of three of these genes can be detected in healthy plants by RNA gel blot analyses. The Atrboh gene products are closely related to gp91phox and the intron locations suggest a common evolutionary origin. A putative EF-hand Ca(2+)-binding motif in the extended N-terminal region of the Atrboh proteins suggests a direct regulatory effect of Ca2+ on the activity of the NADPH oxidase in plants.
据推测，植物防御反应中产生活性氧（ROS）的机制与哺乳动物吞噬细胞中的NADPH氧化酶类似。拟南芥至少含有六个gp91phox同源物，命名为AtrbohA-F（拟南芥呼吸爆发氧化酶同源物），它们位于不同位置。通过RNA凝胶印迹分析可在健康植物中检测到其中三个基因的转录本。Atrboh基因产物与gp91phox密切相关，内含子位置表明它们有共同的进化起源。Atrboh蛋白延伸的N端区域中一个假定的EF手型Ca(2+)结合基序表明Ca2+对植物中NADPH氧化酶的活性有直接调节作用。
