Begum Khaleda, Das Ankur, Ahmed Raja, Akhtar Suraiya, Kulkarni Ram, Banu Sofia
Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, India.
Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune, India.
Front Plant Sci. 2024 Feb 9;14:1326080. doi: 10.3389/fpls.2023.1326080. eCollection 2023.
Respiratory burst oxidase homolog () generates reactive oxygen species (ROS) as a defense response during biotic and abiotic stress. In plants, wounding and fungal infection result in biosynthesis and deposition of secondary metabolites as defense responses, which later form constituents of fragrant resinous agarwood. During injury and fungal invasion, tree generates ROS species via the Rboh enzymes. Despite the implication of genes in agarwood formation, no comprehensive genomic-level study of the gene family in is present. A systematic illustration of their role during stress and involvement in initiating signal cascades for agarwood metabolite biosynthesis is missing. In this study, 14 genes were retrieved from genomes of two species, and , and were classified into five groups. The promoter regions of the genes had abundant of stress-responsive elements. Protein-protein network and expression analysis suggested their functional association with MAPK proteins and transcription factors such as WRKY and MYC2. The study further explored the expression profiles of genes and found them to be differentially regulated in stress-induced callus and stem tissue, suggesting their involvement in ROS generation during stress in . Overall, the study provides in-depth insight into two genes, and , highlighting their role in defense against fungal and abiotic stress, and likely during initiation of agarwood formation through modulation of genes involved in secondary metabolites biosynthesis. The findings presented here offer valuable information about Rboh family members, which can be leveraged for further investigations into ROS-mediated regulation of agarwood formation in species.
呼吸爆发氧化酶同源物（）在生物和非生物胁迫期间产生活性氧（ROS）作为防御反应。在植物中，伤口和真菌感染会导致次生代谢产物的生物合成和沉积作为防御反应，这些次生代谢产物随后形成香脂沉香的成分。在受伤和真菌感染期间，树通过Rboh酶产生活性氧物种。尽管基因在沉香形成中有作用，但目前还没有对该基因家族进行全面的基因组水平研究。它们在胁迫期间的作用以及参与启动沉香代谢产物生物合成信号级联反应的系统阐述尚缺失。在本研究中，从两个物种和的基因组中检索到14个基因，并将其分为五组。这些基因的启动子区域有丰富的胁迫响应元件。蛋白质-蛋白质网络和表达分析表明它们与MAPK蛋白以及WRKY和MYC2等转录因子功能相关。该研究进一步探索了基因的表达谱，发现它们在胁迫诱导的愈伤组织和茎组织中受到差异调节，表明它们参与了胁迫期间的活性氧生成。总体而言，该研究深入了解了两个基因和，突出了它们在抵御真菌和非生物胁迫中的作用，以及可能在通过调节参与次生代谢产物生物合成的基因启动沉香形成过程中的作用。这里呈现的研究结果提供了有关Rboh家族成员的有价值信息，可用于进一步研究ROS介导的沉香形成调控。
