Agarwal H C, Nair A M
Biochem J. 1976 Jul 1;157(1):111-6. doi: 10.1042/bj1570111.
The enzyme cholesterol ester hydrolase (EC 3.1.1.13) was detected in the larvae of the khapra beetle, Trogoderma granarium (Everts). The pH and temperature optima for the enzyme were 6.6 degrees and 37 degrees C respectively. The mol.wt. of the enzyme was 76000-80000. The enzyme was equally effective in hydrolysing cholesteryl acetate, stearate and oleate. Cholesterol derivatives, namely the chloride and the methyl ether, inhibited the enzyme activity almost completely. It was also inhibited completely by p-hydroxymercuribenzoate. This inhibition was reversed by the addition of dithiothreitol, reduced glutathione or cysteine. The enzyme activity was associated predominantly with the 104000 g fraction.
在谷斑皮蠹（Trogoderma granarium (Everts)）幼虫中检测到了胆固醇酯水解酶（EC 3.1.1.13）。该酶的最适pH值和温度分别为6.6和37℃。该酶的分子量为76000 - 80000。该酶在水解醋酸胆固醇、硬脂酸胆固醇和油酸胆固醇方面同样有效。胆固醇衍生物，即氯化物和甲基醚，几乎完全抑制了该酶的活性。对羟基汞苯甲酸也能完全抑制该酶。通过添加二硫苏糖醇、还原型谷胱甘肽或半胱氨酸可逆转这种抑制作用。该酶的活性主要与104000g的组分相关。
