Rabinowitz J L, Lutton C, Chevallier F
Biochem J. 1976 Jul 1;157(1):271-3. doi: 10.1042/bj1570271.
During feeding experiments with [omega-14C]oleic acid and [omega-14c]nervonic acid to adult rats, 14C-labelled C26, C28 and C30 fatty acids were recovered from the intestinal mucosa, liver, plasma, kidney and stools. The structures of these fatty acids were determined by g.l.c., radio-g.l.c. and mass spectrometry. The Schmidt and Ginger degradation methods indicated that most of the 14C found in these extra-long fatty acids remained in the omega position. These radioactive extra-long fatty acids were found mainly in the polar lipids of rats killed 3 or 15 h after being fed on labelled oleic acid or nervonic acid. Rats killed 63 h later yielded only traces of these extra-long fatty acids. When the rats were given antibiotics or received the same radioactive fatty acids by intravenous injection, the labelled extra-long fatty acids could not be detected in any of the tissues. We conclude that they were probably synthesized by elongation of oleic acid and nervonic acid by intestinal micro-organisms (probably yeasts) and then absorbed by the intestinal mucosa.
在用[ω-14C]油酸和[ω-14C]神经酸对成年大鼠进行喂养实验期间，从肠黏膜、肝脏、血浆、肾脏和粪便中回收了14C标记的C26、C28和C30脂肪酸。这些脂肪酸的结构通过气相色谱法、放射性气相色谱法和质谱法确定。施密特和金杰降解方法表明，在这些超长脂肪酸中发现的大部分14C保留在ω位。这些放射性超长脂肪酸主要存在于在喂食标记油酸或神经酸后3小时或15小时处死的大鼠的极性脂质中。63小时后处死的大鼠仅产生这些超长脂肪酸的痕量。当给大鼠使用抗生素或通过静脉注射给予相同的放射性脂肪酸时，在任何组织中均未检测到标记的超长脂肪酸。我们得出结论，它们可能是由肠道微生物（可能是酵母）将油酸和神经酸延长合成，然后被肠黏膜吸收。
