对照大鼠和生长激素处理大鼠肝脏中鸟氨酸脱羧酶的亚细胞定位
Subcellular localization of ornithine decarboxylase in liver of control and growth-hormone-treated rats.
作者信息
Murphy B J, Brosnan M E
出版信息
Biochem J. 1976 Jul 1;157(1):33-9. doi: 10.1042/bj1570033.
Abstract
- Ornithine-2-oxo acid aminotransferase activity was inhibited by amino-oxyacetate (10(-5) M). This permitted the measurement of ornithine decarboxylase in the presence of mitochondria by using the 14CO2-trapping technique. 2. Subcellular fractionation of rat liver by differential centrifugation, followed by the assay of ornithine decarboxylase in the presence of amino oxyacetate and of marker enzymes for each fraction, demonstrated that ornithine decarboxylase was located in the cytosol. 3. The greatly increased ornithine decarboxylase activity observed after growth-hormone administration was also found to be localized in the cytosol. 4. The Km of ornithine decarboxylase from rat liver for ornithine was 28 muM. Administration of growth hormone 4 h before death did not affect the apparent affinity of ornithine decarboxylase for ornithine.
摘要
- 氨基氧乙酸（10⁻⁵ M）可抑制鸟氨酸-2-氧代酸转氨酶的活性。这使得在存在线粒体的情况下，通过使用¹⁴CO₂捕获技术来测量鸟氨酸脱羧酶成为可能。2. 通过差速离心对大鼠肝脏进行亚细胞分级分离，随后在氨基氧乙酸存在的情况下测定鸟氨酸脱羧酶，并对每个分级的标记酶进行测定，结果表明鸟氨酸脱羧酶位于胞质溶胶中。3. 在给予生长激素后观察到的鸟氨酸脱羧酶活性大幅增加也被发现定位于胞质溶胶中。4. 大鼠肝脏鸟氨酸脱羧酶对鸟氨酸的Km值为28 μM。在死亡前4小时给予生长激素并不影响鸟氨酸脱羧酶对鸟氨酸的表观亲和力。
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