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来自荚膜甲基球菌巴斯菌株的一种可溶性甲烷单加氧酶的某些特性。

Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath.

作者信息

Colby J, Dalton H

出版信息

Biochem J. 1976 Aug 1;157(2):495-7. doi: 10.1042/bj1570495.

DOI:10.1042/bj1570495
PMID:962879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163879/
Abstract

Soluble extracts of Methylococcus capsulatus (Bath), obtained by centrifugation of crude extracts at 160000g for 1h, catalyse the NAD(P)H- and O2-dependent disappearance of bromomethane, and also the formation of methanol from methane. Soluble methane mono-oxygenase is not inhibited by chelating agents or by most electron-transport inhibitors, and is a multicomponent enzyme.

摘要

通过将粗提取物在160000g下离心1小时获得的荚膜甲基球菌(巴斯德菌株)的可溶性提取物,可催化NAD(P)H和O₂依赖的溴甲烷消失,以及由甲烷形成甲醇。可溶性甲烷单加氧酶不受螯合剂或大多数电子传递抑制剂的抑制,是一种多组分酶。

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Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath.来自荚膜甲基球菌巴斯菌株的一种可溶性甲烷单加氧酶的某些特性。
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Enrichment, isolation and some properties of methane-utilizing bacteria.甲烷利用菌的富集、分离及某些特性
J Gen Microbiol. 1970 May;61(2):205-18. doi: 10.1099/00221287-61-2-205.
3
Oxidation of C1 Compounds by Particulate fractions from Methylococcus capsulatus: distribution and properties of methane-dependent reduced nicotinamide adenine dinucleotide oxidase (methane hydroxylase).甲基球菌颗粒组分对C1化合物的氧化作用:依赖甲烷的还原型烟酰胺腺嘌呤二核苷酸氧化酶(甲烷羟化酶)的分布及特性
J Bacteriol. 1975 Jun;122(3):1351-63. doi: 10.1128/jb.122.3.1351-1363.1975.
4
Properties and partial purification of the methane-oxidising enzyme system from Methylosinus trichosporium.来自 Trichosporium甲基弯菌的甲烷氧化酶系统的性质及部分纯化
FEBS Lett. 1975 Oct 15;58(1):293-9. doi: 10.1016/0014-5793(75)80282-1.
5
An improved assay for bacterial methane mono-oxygenase: some properties of the enzyme from Methylomonas methanica.一种改进的细菌甲烷单加氧酶检测方法:甲基单胞菌属甲烷菌中该酶的一些特性
Biochem J. 1975 Nov;151(2):459-62. doi: 10.1042/bj1510459.
6
Oxidation of carbon monoxide and methane by Pseudomonas methanica.甲烷假单胞菌对一氧化碳和甲烷的氧化作用。
J Gen Microbiol. 1975 Nov;91(1):79-91. doi: 10.1099/00221287-91-1-79.