一种改进的细菌甲烷单加氧酶检测方法：甲基单胞菌属甲烷菌中该酶的一些特性
An improved assay for bacterial methane mono-oxygenase: some properties of the enzyme from Methylomonas methanica.
作者信息
Colby J, Dalton H, Whittenbury R
出版信息
Biochem J. 1975 Nov;151(2):459-62. doi: 10.1042/bj1510459.
Abstract
Extracts of Methylomonas methanica catalyse the O2-and NAD(P)H-dependent disappearance of bromomethane. The activity is unstable at 2 degrees C but is stable at --70 degrees C for several weeks. Bromomethane mono-oxygenase is particulate and is inhibited by metal-binding reagents, by compounds SKF 525A and Lilly 53325, by some metal ions and by acetylene. Evidence is presented that indicates that bromomethane mono-oxygenase is the enzyme responsible for methane oxidation in vivo.
摘要
甲基单胞菌提取物催化溴甲烷依赖氧气和NAD（P）H的消失反应。该活性在2℃不稳定，但在-70℃下可稳定数周。溴甲烷单加氧酶存在于颗粒中，可被金属结合试剂、化合物SKF 525A和礼来53325、某些金属离子以及乙炔抑制。有证据表明，溴甲烷单加氧酶是体内负责甲烷氧化的酶。
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