Ferenci T, Strom T, Quayle J R
J Gen Microbiol. 1975 Nov;91(1):79-91. doi: 10.1099/00221287-91-1-79.
The oxidation of carbon monoxide and methane by suspensions and ultrasonic extracts of Pseudomonas methanica was studied. A continuous assay for the oxidation of CO to CO2 was devised, using O2 and CO2 electrodes in combination. Stoicheiometries of CO-dependent CO2 formation, O2 consumption and NADH oxidation, and the partial stoicheiometries of methane-dependent NADH oxidation, suggest the involvement of a mono-oxygenase in these oxidations. Evidence is presented suggesting methane and CO oxidation are catalysed by a single enzyme system, distinct, at least in part, from the NADH oxidase present in extracts. Ethanol was able to provide the reductant necessary for CO oxidation by cell suspensions, though the metabolism of ethanol by P. methanica was found unlikely to result in substrate-level formation of NADH; the means whereby alcohol oxidation could supply reductant for the mono-oxygenase are discussed.
研究了甲烷假单胞菌的悬浮液和超声提取物对一氧化碳和甲烷的氧化作用。设计了一种连续测定法，使用氧气和二氧化碳电极组合，将一氧化碳氧化为二氧化碳。一氧化碳依赖的二氧化碳形成、氧气消耗和NADH氧化的化学计量，以及甲烷依赖的NADH氧化的部分化学计量，表明单加氧酶参与了这些氧化反应。有证据表明，甲烷和一氧化碳的氧化是由单一酶系统催化的，该系统至少部分不同于提取物中存在的NADH氧化酶。乙醇能够为细胞悬浮液氧化一氧化碳提供所需的还原剂，尽管发现甲烷假单胞菌对乙醇的代谢不太可能导致底物水平的NADH形成；讨论了酒精氧化为单加氧酶提供还原剂的方式。
