胃蛋白酶抑制剂与猪胃蛋白酶原的相互作用。
Interaction of pepstatin with pig pepsinogen.
作者信息
Kay J, Dykes C W
出版信息
Biochem J. 1976 Aug 1;157(2):499-502. doi: 10.1042/bj1570499.
Abstract
In contrast with pepsin, pepsinogen does not bind pepstatin at pH values between 5.3 and 2.5. Pepsinogen is not retarded by pepstatin immobilized on to aminohexyl-Sepharose at pH5.3 or 4.1, whereas at pH3.0 activation takes place during the chromatography, with retardation of the resultant pepsin.
摘要
与胃蛋白酶相比，胃蛋白酶原在pH值5.3至2.5之间不与胃蛋白酶抑制剂结合。在pH5.3或4.1时，固定在氨基己基琼脂糖上的胃蛋白酶抑制剂不会使胃蛋白酶原滞留，而在pH3.0时，在色谱过程中会发生激活，生成的胃蛋白酶会滞留。
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