Chaulk S G, MacMillan A M
Department of Chemistry, University of Toronto, Toronto, Ontario M5S-3HC, Canada.
Nucleic Acids Res. 1998 Jul 1;26(13):3173-8. doi: 10.1093/nar/26.13.3173.
We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and dynamics.
我们在此报告了通过用笼蔽官能团对锤头状系统中2'-羟基亲核试剂进行位点特异性修饰，首次实现了对核酶反应的光化学控制。在天然条件下，O-(2-硝基苄基)笼蔽基团的快速激光光解引发了底物RNA高效且准确的锤头状催化切割。以这种方式将反应性官能团或识别元件进行笼蔽的RNA，将成为探测RNA反应性和动力学的有用工具。
