乳清酸喂养诱导的脂肪肝中类固醇的代谢
The metabolism of steroids in the fatty liver induced by orotic acid feeding.
作者信息
Carrella M, Björkhem I, Gustafsson J A, Einarsson K, Hellström K
出版信息
Biochem J. 1976 Jul 15;158(1):89-95. doi: 10.1042/bj1580089.
Abstract
- The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5alpha-[4-14C]androstane-3alpha,17beta-diol, [4-14C]cholesterol, 7alpha-hydroxy-4-[6beta-3H]cholesten-3-one, 5beta-[7beta-3H]cholestane-3alpha,7alpha-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 6beta- and 7alpha-hydroxylation of 4-androstene3,17-dione, and the 2alpha-, 2beta- and 18-hydroxylation of 5alpha-androstane-3alpha,17beta-diol, and the 5alpha-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40--50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one was decreased by about 50%, whereas the 7alpha-hydroxylation of cholesterol and the 26-hydroxylation of 5beta-cholestane-3alpha,7alpha-diol were not significantly decreased. The 6beta-hydroxylation of lithocholic acid was stimulated by 40%. 5. The results are discussed in relation to present knowledge of the heapatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.
摘要
- 研究了4-[4-¹⁴C]雄甾烯-3,17-二酮、4-[4-¹⁴C]孕烯-3,20-二酮、5α-[4-¹⁴C]雄甾烷-3α,17β-二醇、[4-¹⁴C]胆固醇、7α-羟基-4-[6β-³H]胆甾烯-3-酮、5β-[7β-³H]胆甾烷-3α,7α-二醇和[³H]石胆酸在对照和乳清酸处理的雄性大鼠肝脏微粒体部分的代谢。2. 作为处理的结果，喂食乳清酸的大鼠出现脂肪肝，血清中胆固醇和甘油三酯浓度低于正常水平。3. 在喂食乳清酸的大鼠中，4-雄甾烯-3,17-二酮的6β-和7α-羟基化、5α-雄甾烷-3α,17β-二醇的2α-、2β-和18-羟基化以及4-雄甾烯-3,17-二酮和4-孕烯-3,20-二酮的5α-还原减少了40%-50%。甾体激素的其他氧化和还原反应未受到显著影响。4. 7α-羟基-4-胆甾烯-3-酮的12α-羟基化减少了约50%，而胆固醇的7α-羟基化和5β-胆甾烷-3α,7α-二醇的26-羟基化未显著减少。石胆酸的6β-羟基化增加了40%。5. 结合目前关于肝脏药物代谢酶的知识以及肝病中胆汁酸代谢异常的最新发现对结果进行了讨论。
相似文献
1
The metabolism of steroids in the fatty liver induced by orotic acid feeding.乳清酸喂养诱导的脂肪肝中类固醇的代谢
Biochem J. 1976 Jul 15;158(1):89-95. doi: 10.1042/bj1580089.
2
Influence of cholesterol feeding on liver microsomal metabolism of steroids and bile acids in conventional and germ-free rats.胆固醇喂养对普通大鼠和无菌大鼠肝脏微粒体中类固醇及胆汁酸代谢的影响。
J Biol Chem. 1975 Nov 10;250(21):8496-502.
3
Influence of fructose feeding on individual enzymic reactions in the formation and metabolism of bile acids in rat liver homogenates.果糖喂养对大鼠肝脏匀浆中胆汁酸形成和代谢过程中各个酶促反应的影响。
Br J Nutr. 1976 Sep;36(2):273-9. doi: 10.1079/bjn19760079.
4
Studies on the 12alpha and 26-hydroxylation of bile alcohols by rabbit liver microsomes.兔肝微粒体对胆汁醇的12α和26-羟基化作用的研究。
Lipids. 1976 Feb;11(2):148-52. doi: 10.1007/BF02532665.
5
Effect of biliary obstruction on 26-hydroxylation of C27-steroids in bile acid synthesis.胆汁淤积对胆汁酸合成中C27-类固醇26-羟化作用的影响。
J Lipid Res. 1978 Feb;19(2):237-43.
6
Partial feminization of hepatic steroid metabolism in male rats after neonatal administration of cyproterone acetate.新生期给予醋酸环丙孕酮后雄性大鼠肝脏类固醇代谢的部分女性化
J Endocrinol. 1975 Feb;64(2):267-75. doi: 10.1677/joe.0.0640267.
7
Effects of clofibrate on some microsomal hydroxylations involved in the formation and metabolism of bile acids in rat liver.氯贝丁酯对大鼠肝脏中参与胆汁酸形成和代谢的某些微粒体羟化作用的影响。
Biochem J. 1976 May 15;156(2):445-8. doi: 10.1042/bj1560445.
8
Feminization of hepatic steroid metabolism in male rats following electrothermic lesion of the hypothalamus.下丘脑电热损伤后雄性大鼠肝脏类固醇代谢的雌性化
Endocrinology. 1976 Apr;98(4):922-6. doi: 10.1210/endo-98-4-922.
9
Partial masculinization of rat liver enzyme activities following treatment with FSH.用促卵泡激素处理后大鼠肝脏酶活性的部分雄性化
Endocrinology. 1975 Feb;96(2):501-4. doi: 10.1210/endo-96-2-501.
10
Formation of bile acids in man: conversion of cholesterol into 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol in liver homogenates.人体内胆汁酸的形成：肝脏匀浆中胆固醇向5-β-胆甾烷-3-α,7-α,12-α-三醇的转化
J Clin Invest. 1968 Jul;47(7):1573-82. doi: 10.1172/JCI105849.
引用本文的文献
1
Sea Cucumber Saponins Derivatives Alleviate Hepatic Lipid Accumulation Effectively in Fatty Acids-Induced HepG2 Cells and Orotic Acid-Induced Rats.海参皂苷衍生物有效缓解脂肪酸诱导的 HepG2 细胞和鸟氨酸诱导的大鼠肝脂质蓄积。
Mar Drugs. 2022 Nov 10;20(11):703. doi: 10.3390/md20110703.
本文引用的文献
1
BIOCHEMICAL AND NUTRITIONAL STUDIES ON THE INDUCTION OF FATTY LIVERS BY DIETARY OROTIC ACID.关于膳食乳清酸诱导脂肪肝的生化与营养研究
Proc Natl Acad Sci U S A. 1960 Feb;46(2):178-86. doi: 10.1073/pnas.46.2.178.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
AN OROTIC ACID-INDUCED, ADENINE-REVEREED INHIBITION OF HEPATIC LIPOPROTEIN SECRETION IN THE RAT.乳清酸诱导、腺嘌呤逆转的大鼠肝脏脂蛋白分泌抑制作用
J Biol Chem. 1964 Feb;239:530-7.
4
Fatty livers induced by orotic acid. I. Accumulation and metabolism of lipids.
J Biol Chem. 1961 Jul;236:2064-70.
5
Fatty liver induced by orotic acid feeding.由乳清酸喂养诱导的脂肪肝。
Proc Soc Exp Biol Med. 1955 Oct;90(1):270-1. doi: 10.3181/00379727-90-22005.
6
Effect of carbon monoxide and phenobarbital on hydroxylation of bile acids by rat liver microsomes.一氧化碳和苯巴比妥对大鼠肝微粒体胆汁酸羟化作用的影响。
FEBS Lett. 1969 Aug;4(3):177-180. doi: 10.1016/0014-5793(69)80228-0.
7
Effect of biliary drainage on individual reactions in the conversion of cholesterol to taurochlic acid. Bile acids and steroids 180.胆汁引流对胆固醇转化为牛磺胆酸过程中个体反应的影响。胆汁酸与类固醇180。
Eur J Biochem. 1967 Jul;2(1):44-9. doi: 10.1111/j.1432-1033.1967.tb00103.x.
8
Total inhibition of hepatic beta-lipoprotein production in the rat by orotic acid.乳清酸对大鼠肝脏β-脂蛋白生成的完全抑制作用。
J Biol Chem. 1967 May 10;242(9):2246-54.
9
Effect of dietary orotic acid and adenine sulfate on hepatic microsomal enzymes in male and female rats.
Biochem Pharmacol. 1969 Aug;18(8):1922-33. doi: 10.1016/0006-2952(69)90288-3.
10
Steroids in newborns and infants identification by combined gas chromatography-mass spectrometry of the major steroids excreted by a newborn chimpanzee (Pan troglodytes).
Steroids. 1971 Aug;18(2):175-86. doi: 10.1016/0039-128x(71)90024-9.
Zotero 插件