果蝇切割基因座中的论坛结构域内部存在环状结构域。
Forum domain in Drosophila melanogaster cut locus possesses looped domains inside.
作者信息
Tchurikov N A, Krasnov A N, Ponomarenko N A, Golova Y B, Chernov B K
机构信息
Department of Genome Organization and Group of Genes Chemical Synthesis, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov str. 32, Moscow B334, 117984, Russia.
出版信息
Nucleic Acids Res. 1998 Jul 1;26(13):3221-7. doi: 10.1093/nar/26.13.3221.
We have studied the relationship between chromosomal forum domains and looped domains in the cut locus of Drosophila melanogaster . Forum domains were earlier detected by separation in pulsed-field gels of 50-150 kb chromosomal DNA fragments obtained after spontaneous non-random degradation of chromosomes. We have localized the boundary region where cleavage sites are scattered between two forum domains in the regulatory region of the cut locus. We have sequenced a 13 kb region spanning few kilobases from distal domain, the boundary region and part of the proximal forum domain where several scaffold associated regions (SARs) were observed. We conclude that forum domains and looped domains are physically different types of domains and belong to different levels of organization in eukaryotic chromosomes. The boundary region between the neighboring forum domains in the cut locus possesses the Doc element insertion and a micro-satellite stretch and thus might remind a small island of heterochromatin and correspond to so-called intercalary heterochromatin that is known to be located in the 7B1-2 band where the major part of the cut locus is reside.
我们研究了黑腹果蝇cut基因座切割位点处染色体结构域与环状结构域之间的关系。结构域是通过对染色体自发非随机降解后获得的50 - 150 kb染色体DNA片段进行脉冲场凝胶分离而较早检测到的。我们已经定位了切割基因座调控区域中两个结构域之间切割位点分散的边界区域。我们对一个13 kb的区域进行了测序，该区域跨越了远端结构域的几千个碱基、边界区域以及近端结构域的一部分，在近端结构域中观察到了几个支架相关区域（SARs）。我们得出结论，结构域和环状结构域在物理上是不同类型的结构域，属于真核染色体中不同层次的组织。cut基因座中相邻结构域之间的边界区域具有Doc元件插入和微卫星延伸，因此可能类似于异染色质的一个小岛，对应于所谓的居间异染色质，已知其位于7B1 - 2带，cut基因座的大部分位于该带。
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