Brookes P S, Buckingham J A, Tenreiro A M, Hulbert A J, Brand M D
Department of Biochemistry, University of Cambridge, UK.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Feb;119(2):325-34. doi: 10.1016/s0305-0491(97)00357-x.
We measured the proton leak across the inner membrane of liver mitochondria isolated from six different vertebrate species and from obese and control Zucker rats. Proton leak at 37 degrees C was similar in rat and pigeon, and in obese and control Zucker rats. Compared to rat, it was lower in cane toad, shingleback lizard, and the Madeiran lizard Lacerta dugessi. Proton leak at 20 degrees C was similar in xenopus toad and higher in rainbow trout, compared to rat. In general, proton permeability and substrate oxidation activity were greater in liver mitochondria from endotherms than those from ectotherms. Analysis of this and previous data showed that proton leak per milligram of mitochondrial protein correlated with standard metabolic rate, and proton leak per milligram of inner membrane phospholipid correlated with 11 phospholipid fatty acid compositional parameters, including unsaturation index.
我们测量了从六种不同脊椎动物物种以及肥胖和对照的 Zucker 大鼠分离出的肝脏线粒体内膜的质子泄漏情况。37 摄氏度时，大鼠和鸽子以及肥胖和对照的 Zucker 大鼠的质子泄漏情况相似。与大鼠相比，甘蔗蟾蜍、松果蜥和马德拉蜥蜴（Lacerta dugessi）的质子泄漏较低。20 摄氏度时，非洲爪蟾的质子泄漏与大鼠相似，虹鳟鱼的质子泄漏比大鼠高。一般来说，恒温动物肝脏线粒体的质子通透性和底物氧化活性比变温动物的更大。对这些数据和之前数据的分析表明，每毫克线粒体蛋白的质子泄漏与标准代谢率相关，每毫克内膜磷脂的质子泄漏与 11 个磷脂脂肪酸组成参数相关，包括不饱和度指数。
