McDonnell G V, McMillan S A, Douglas J P, Droogan A G, Hawkins S A
Northern Ireland Neurology Service, Royal Victoria Hospital, Belfast, UK.
J Neuroimmunol. 1998 May 15;85(2):186-92. doi: 10.1016/s0165-5728(98)00009-5.
Endothelial activation is considered an important step in multiple sclerosis (MS) lesion formation, elevated cerebrospinal fluid (CSF) and serum levels of certain adhesion molecules being associated with varying stages of disease activity and clinical course. CSF and serum sVCAM-1, sICAM-1, sE-selectin and sL-selectin were measured by ELISA in 16 primary progressive (PPMS), 16 secondary progressive (SPMS) and 43 relapsing-remitting MS patients (RRMS) and compared with 20 inflammatory (IND) and 46 non-inflammatory neurological disease (NIND) controls. CSF sVCAM-1 and sICAM-1 were increased in all MS groups vs. NIND with no significant differences between the MS groups. CSF sE-selectin (p = 0.007) and the sE-selectin index (p = 0.01) were elevated in PPMS vs. RRMS in relapse, whilst serum sE-selectin was significantly raised in PPMS compared to RRMS in remission (p = 0.005), RRMS in relapse (p = 0.004), NIND (p = 0.03) and IND (p = 0.05). Adhesion molecule levels in both progressive MS groups were similar. These results provide evidence for a distinct inflammatory component in PPMS and for immunological heterogeneity between the clinical subgroups of MS.
内皮细胞活化被认为是多发性硬化症（MS）病灶形成的重要步骤，脑脊液（CSF）和血清中某些黏附分子水平升高与疾病活动和临床病程的不同阶段相关。通过酶联免疫吸附测定（ELISA）法检测了16例原发进展型（PPMS）、16例继发进展型（SPMS）和43例复发缓解型MS患者（RRMS）的脑脊液和血清中可溶性血管细胞黏附分子-1（sVCAM-1）、可溶性细胞间黏附分子-1（sICAM-1）、可溶性E-选择素（sE-selectin）和可溶性L-选择素（sL-selectin），并与20例炎症性（IND）和46例非炎症性神经系统疾病（NIND）对照进行比较。与NIND相比，所有MS组的脑脊液sVCAM-1和sICAM-1均升高，MS组之间无显著差异。复发时，PPMS组的脑脊液sE-选择素（p = 0.007）和sE-选择素指数（p = 0.01）高于RRMS组，而缓解期PPMS组的血清sE-选择素显著高于RRMS组（p = 0.005）、复发期RRMS组（p = 0.004）、NIND组（p = 0.03）和IND组（p = 0.05）。两个进展型MS组的黏附分子水平相似。这些结果为PPMS中存在独特的炎症成分以及MS临床亚组之间的免疫异质性提供了证据。
