Droogan A G, McMillan S A, Douglas J P, Hawkins S A
Department of Neurology, Royal Victoria Hospital, Belfast, UK.
J Neuroimmunol. 1996 Feb;64(2):185-91. doi: 10.1016/0165-5728(95)00174-3.
Activated cerebral vascular endothelial cells express leukocyte, vascular cell, and intracellular adhesion molecules (E-selectin, VCAM-1 and ICAM-1) which facilitate leukocyte adhesion to endothelium and migration into inflammatory lesions. Paired serum and cerebrospinal fluid (CSF) levels of soluble (s) E-selectin, sVCAM-1 and sICAM-1 were determined by ELISA in patients with clinically definite MS in relapse, and patients with other inflammatory (IND) and non-inflammatory neurological disease (NIND). CSF levels of sVCAM-1 and sICAM-1 were significantly increased in MS patients compared to IND and NIND patients. Elevation of CSF sVCAM-1 in MS patients was the most marked finding (P = 0.0001) and an increased sVCAM-1 index indicated that this was due to intrathecal release of sVCAM-1. There were no differences in serum and CSF sE-selectin levels between the study groups. Measurement of the sVCAM-1 index may provide a marker of disease activity in patients with clinically definite MS.
活化的脑血管内皮细胞表达白细胞、血管细胞和细胞内黏附分子（E-选择素、血管细胞黏附分子-1和细胞间黏附分子-1），这些分子有助于白细胞黏附于内皮并迁移至炎症病灶。采用酶联免疫吸附测定法（ELISA）检测复发期临床确诊多发性硬化症（MS）患者、其他炎症性疾病（IND）患者和非炎症性神经疾病（NIND）患者血清和脑脊液（CSF）中可溶性（s）E-选择素、s血管细胞黏附分子-1和s细胞间黏附分子-1的水平。与IND和NIND患者相比，MS患者CSF中s血管细胞黏附分子-1和s细胞间黏附分子-1水平显著升高。MS患者CSF中s血管细胞黏附分子-1升高最为明显（P = 0.0001），s血管细胞黏附分子-1指数升高表明这是由于s血管细胞黏附分子-1的鞘内释放所致。研究组之间血清和CSF中sE-选择素水平无差异。检测s血管细胞黏附分子-1指数可能为临床确诊MS患者的疾病活动提供一个标志物。
