Zetterström T S, Pei Q, Grahame-Smith D G
Oxford University-SmithKline Beecham Centre for Applied Neuropsychobiology, University Department of Clinical Pharmacology, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UK.
Brain Res Mol Brain Res. 1998 Jun 1;57(1):106-10. doi: 10.1016/s0169-328x(98)00077-1.
Brain-derived neurotrophic factor (BDNF) is known to modulate synaptic function as well as to promote neuronal growth in the adult brain. The aim of the present study was to compare the duration of electroconvulsive shock (ECS)-induced BDNF gene expression following a single shock (acute ECS) to the more clinically relevant situation, where repeated shocks (chronic ECS) are administered. For this purpose, we have used quantitative in situ hybridisation with a 35S-labelled oligonucleotide probe complementary to mRNAs encoding genes for all forms of BDNF. The results confirm previous studies that the administration of ECS increases BDNF mRNA abundance in parts of rat brain with particularly marked changes in the granule cell layer of the dentate gyrus. We also for the first time show the long lasting nature of the increase in BDNF mRNA abundance measured after chronic ECS, i.e., significant increases in BDNF mRNA persisted up to 48 h after the last shock. Acute ECS at 6 h after the shock produced a slightly more pronounced effect on BDNF mRNA abundance than chronic ECS 6 h after the last shock. However, this change was not detectable already 24 h after a single ECS. These results indicate that repeated ECS induces adaptive changes in BDNF mRNA expression.
脑源性神经营养因子（BDNF）已知可调节突触功能，并促进成人大脑中的神经元生长。本研究的目的是比较单次电击（急性电惊厥休克，ECS）诱导的BDNF基因表达持续时间与更具临床相关性的重复电击（慢性ECS）情况。为此，我们使用了与编码所有形式BDNF基因的mRNA互补的35S标记寡核苷酸探针进行定量原位杂交。结果证实了先前的研究，即ECS的施用会增加大鼠脑部分区域中BDNF mRNA的丰度，在齿状回颗粒细胞层中变化尤为明显。我们还首次显示了慢性ECS后测量的BDNF mRNA丰度增加的持久性，即最后一次电击后48小时内BDNF mRNA仍显著增加。电击后6小时的急性ECS对BDNF mRNA丰度的影响比最后一次电击后6小时的慢性ECS略明显。然而，单次ECS后24小时就无法检测到这种变化。这些结果表明，重复ECS会诱导BDNF mRNA表达的适应性变化。
