Taylor N J, Edwards M, Kiernan R J, Davey C D, Blakesley D, Henshaw G G
School of Biology and Biochemistry, University of Bath, UK.
Nat Biotechnol. 1996 Jun;14(6):726-30. doi: 10.1038/nbt0696-726.
Procedures for the production of a new and highly prolific embryogenic culture system have been developed in cassava. The importance of the basal salts and type of auxin in controlling the development of cassava embryogenic tissues has been demonstrated, with culture on Gresshoff and Doy basal medium in the presence of 4-amino-3,5,6,trichloro-picolinic acid (picloram) inducing the formation of friable embryogenic callus from which highly totipotent embryogenic suspension cultures could be established. Plants have been regenerated from these cultures. The availability of embryogenic suspension cultures is considered to have important implications for the application of genetic transformation and other biotechnologies in the agronomic improvement of cassava.
木薯已开发出生产新的高产胚性培养系统的程序。已证明基本盐类和生长素类型在控制木薯胚性组织发育中的重要性，在4-氨基-3,5,6-三氯吡啶甲酸（毒莠定）存在下，在格雷斯霍夫和多伊基本培养基上培养可诱导形成易碎胚性愈伤组织，从中可建立高度全能的胚性悬浮培养物。已从这些培养物中再生出植株。胚性悬浮培养物的可用性被认为对基因转化和其他生物技术在木薯农艺改良中的应用具有重要意义。
