[亚细胞颗粒膜的跨膜电位差。II. 测量的补偿方法]
[Potential difference across subcellular particle membranes. II. Compensation method of measurement].
作者信息
Liberman E A, Arzumanian A M, Vladimirova M A, Tsofina L M
出版信息
Biofizika. 1976 May-Jun;21(3):476-81.
"Zero-loop" of the molecular potential transformer of submitochondrial particles (SMP) is separated from the remaining electron transfer chain by rotenone, and its e.m.f. ET=0,003+RT/2F in [NADP X H] [NAD+]/[NADP+] [NAD X H] volts is used in the compensative method of measurement of the potential difference across the SMP membrane (delta USMP). The phospholipid membrane, measuring the concentration of the penetrating anions in the solution contained SMP, is used as "zero-indicators". This concentration drops monotonically with increase in delta USMP. Delta USMP is equal to ET when the addition of substrates of transhydrogenase reaction with definite ET does not change the potential across phospholipid membrane.
亚线粒体颗粒（SMP）分子电势转换器的“零环”被鱼藤酮与其余电子传递链分隔开，其电动势ET = 0.003 + RT/2F，单位为伏特，其中[NADP X H][NAD+]/[NADP+][NAD X H]，该电动势用于补偿测量SMP膜上电势差（δUSMP）的方法中。磷脂膜用于测量含有SMP的溶液中穿透性阴离子的浓度，作为“零指示器”。随着δUSMP的增加，该浓度单调下降。当加入具有确定ET的转氢酶反应底物不会改变磷脂膜上的电势时，δUSMP等于ET。
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