Blechl A E, Anderson O D
Agricultural Research Service, US Department of Agriculture, Albany, CA 94710-1105, USA.
Nat Biotechnol. 1996 Jul;14(7):875-9. doi: 10.1038/nbt0796-875.
High-molecular-weight glutenin subunits (HMW-GS), one class of seed storage proteins, are important determinants of the bread-making quality of wheat flour. To change the amount and composition of these proteins via genetic engineering, a gene encoding a novel hybrid subunit under the control of native HMW-GS regulatory sequences was inserted into wheat. Of 26 independent transgenic lines identified by bialaphos selection, 18 expressed the cotransformed hybrid HMW-GS gene in their seed. The hybrid subunit accumulated to levels comparable to those of the native HMW-GS. These results show that a native HMW-GS gene promoter can be used to obtain high levels of expression of seed storage and, potentially, other proteins in transgenic wheat endosperm. Transgene expression was stable for at least three seed generations in the majority of lines. These experiments demonstrate the feasibility of constructing wheat plants with novel seed protein compositions.
高分子量谷蛋白亚基（HMW-GS）是一类种子贮藏蛋白，是小麦面粉面包制作品质的重要决定因素。为了通过基因工程改变这些蛋白质的数量和组成，将一个在天然HMW-GS调控序列控制下编码新型杂交亚基的基因导入小麦。通过双丙氨膦筛选鉴定出的26个独立转基因株系中，有18个在其种子中表达了共转化的杂交HMW-GS基因。杂交亚基的积累水平与天然HMW-GS相当。这些结果表明，天然HMW-GS基因启动子可用于在转基因小麦胚乳中高水平表达种子贮藏蛋白以及潜在的其他蛋白质。在大多数株系中，转基因表达在至少三代种子中保持稳定。这些实验证明了构建具有新型种子蛋白组成的小麦植株的可行性。
