Atabay H I, Corry J E
University of Bristol, Department of Clinical Veterinary Science, Langford, UK.
Int J Food Microbiol. 1998 May 5;41(1):53-8. doi: 10.1016/s0168-1605(98)00034-8.
The productivity of an arcobacter enrichment medium (AM), newly developed by Oxoid was compared with two campylobacter enrichment media (Preston broth (Oxoid) and LabM broth), with arcobacter basal medium (ABM) as control. Twenty strains of Arcobacter and Campylobacter spp. were tested for growth, with target inocula of < 4 cfu per ml of medium. Incubation was carried out aerobically for 48 h with tightly closed caps at 25 degrees C for arcobacters and 37 degrees C for campylobacters. After incubation the numbers of cfu in the broths were counted by surface-plating on blood agar. None of the Campylobacter spp. grew in the complete AM, and only one grew (very poorly) in the arcobacter basal medium. However, AM supported good growth of all three species of Arcobacter (A. butzleri, A. skirrowii and A. cryaerophilus) which have been associated with human and animal disease. The one strain of A. nitrofigilis tested failed to grow in any of the media except poorly in ABM. None of the Arcobacter spp. grew in Preston Broth, but nine grew in LabM broth, although productivity was poor compared to AM. None of the Campylobacter spp. grew in AM and only one grew (very poorly) in ABM.
对Oxoid新开发的一种弓形杆菌增菌培养基（AM）与两种弯曲杆菌增菌培养基（普雷斯顿肉汤（Oxoid）和LabM肉汤）进行了比较，并以弓形杆菌基础培养基（ABM）作为对照。对20株弓形杆菌和弯曲杆菌菌株进行了生长测试，目标接种量为每毫升培养基<4 cfu。弓形杆菌在25℃下需氧培养48小时，培养瓶瓶盖紧闭；弯曲杆菌在37℃下培养。培养后，通过在血琼脂上进行表面接种来计算肉汤中的cfu数量。所有弯曲杆菌菌株在完全AM中均未生长，只有一株在弓形杆菌基础培养基中生长（非常差）。然而，AM支持与人和动物疾病相关的所有三种弓形杆菌（布氏弓形杆菌、斯氏弓形杆菌和嗜冷弓形杆菌）良好生长。所测试的一株硝化弓形杆菌在除ABM外的任何培养基中均未生长，在ABM中生长也很差。没有弓形杆菌菌株在普雷斯顿肉汤中生长，但有九株在LabM肉汤中生长，不过与AM相比，其生长效率较低。所有弯曲杆菌菌株在AM中均未生长，只有一株在ABM中生长（非常差）。
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