Weide-Botjes M, Kobe B, Lange C, Schwarz S
Institut für Tierzucht und Tierverhalten, Bundesforschungsanstalt für Landwirtschaft Braunschweig-Völkenrode (FAL), Celle, Germany.
Vet Microbiol. 1998 Mar 31;61(3):215-27. doi: 10.1016/s0378-1135(98)00181-3.
A highly discriminatory molecular typing system for isolates of Salmonella enterica subsp. enterica (S.) serovar Hadar was developed based on the data obtained from 34 epidemiologically unrelated S. Hadar isolates from Germany and Brazil. Plasmid profile analysis and macrorestriction analysis with the enzymes XbaI, SpeI and BlnI represented the most discriminative typing methods. In contrast, ribotyping as performed with a gene probe that recognized the entire rrn operon did not reveal any differences between the S. Hadar isolates. IS200 typing was not applicable due to the lack of insertion elements of this type in all S. Hadar isolates included in this study. The combination of the results of plasmid analysis and macrorestriction analysis subdivided the 34 S. Hadar isolates into 32 different genomic groups. The high discriminatory power of these methods confirmed their suitability as effective typing methods for epidemiological studies on S. Hadar isolates. The practical application of this molecular typing system to S. Hadar isolates of the same flock obtained during a 15 month period strongly suggested recurring introduction of genomically different S. Hadar isolates instead of a single introduction of S. Hadar followed by subsequent spread within the flock.
基于从德国和巴西的34株流行病学上无关联的肠炎沙门氏菌亚种肠炎血清型哈达尔菌株获得的数据，开发了一种高度区分性的分子分型系统。质粒图谱分析以及用XbaI、SpeI和BlnI酶进行的宏观限制性分析代表了最具区分性的分型方法。相比之下，使用识别整个rrn操纵子的基因探针进行的核糖体分型未显示哈达尔菌株之间存在任何差异。由于本研究中纳入的所有哈达尔菌株均缺乏此类插入元件，因此IS200分型不适用。质粒分析和宏观限制性分析结果的结合将34株哈达尔菌株细分为32个不同的基因组群。这些方法的高区分能力证实了它们适合作为哈达尔菌株流行病学研究的有效分型方法。该分子分型系统在15个月期间对同一鸡群的哈达尔菌株的实际应用强烈表明，基因组不同的哈达尔菌株是反复引入的，而不是单次引入哈达尔菌株后在鸡群中随后传播。
