系统性红斑狼疮患者外周血T细胞共刺激分子CD80的表达

Expression of costimulatory molecule CD80 on peripheral blood T cells in patients with systemic lupus erythematosus.

作者信息

Takasaki Y, Ogaki M, Abe K, Takeuchi K, Ando S, Tokano Y, Kobayashi S, Sekigawa I, Tsuda H, Hashimoto H

机构信息

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Mitsubishi Chemical Corporation, Tokyo, Japan.

出版信息

J Rheumatol. 1998 Jun;25(6):1085-91.

PMID:9632068

Abstract

OBJECTIVE

To investigate the expression of costimulatory molecule CD80 on T cells of peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE).

METHODS

Monoclonal antibodies against CD80 were used for flow cytometry and expression of CD80 on PBMC was studied in 26 patients with SLE, 18 patients with rheumatoid arthritis (RA), 8 patients with mixed connective tissue disease (MCTD), and 22 healthy controls.

RESULTS

CD80 was detected on CD3+ and CD19+ cells in patients with SLE and it was significantly higher than that of controls. In patients with SLE CD80 was expressed on CD4+ T cells (8.05+/-5.45%), significantly higher than in RA and controls, but was not highly expressed on CD8+ T cells (1.67+/-2.87%). CD80+CD4+ T cell phenotype analysis revealed CD45RA-, CD45RO+, and CD25+, or HLA-DR+ activated T cells. The percentage of CD80+ cells in CD4+ cells increased in the active stage of SLE, and was significantly correlated with the SLE disease activity index.

CONCLUSION

CD80 can be expressed on activated CD4+ T cells in PBMC of patients with SLE in vivo and the appearance of these cells is associated with the disease activity in SLE.

摘要

目的

研究系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中T细胞共刺激分子CD80的表达情况。

方法

采用抗CD80单克隆抗体进行流式细胞术检测，研究26例SLE患者、18例类风湿关节炎（RA）患者、8例混合性结缔组织病（MCTD）患者及22名健康对照者PBMC中CD80的表达。

结果

SLE患者的CD3⁺和CD19⁺细胞上可检测到CD80，且显著高于对照组。SLE患者CD4⁺T细胞上表达CD80（8.05±5.45%），显著高于RA患者及对照组，但在CD8⁺T细胞上表达不高（1.67±2.87%）。CD80⁺CD4⁺T细胞表型分析显示为CD45RA⁻、CD45RO⁺、CD25⁺或HLA-DR⁺活化T细胞。SLE活动期CD4⁺细胞中CD80⁺细胞百分比增加，且与SLE疾病活动指数显著相关。