Sender S, Decker B, Fenske C D, Sly W S, Carter N D, Gros G
Vegetative Physiologie, Zentrum Physiologie, Medizinische Hochschule Hannover, Hannover, Germany.
J Histochem Cytochem. 1998 Jul;46(7):855-61. doi: 10.1177/002215549804600709.
We investigated carbonic anhydrase IV (CA IV) in rat and human heart with immunohistochemical methods by both light and electron microscopy. In cryosections that were incubated with anti-CA IV/FITC, the capillaries showed a strong reaction for CA IV. In paraffin and semithin sections treated with anti-CA IV/ABC (avidin-biotin-peroxidase complex) blood vessels, capillaries, and sarcolemma (SL) were positively stained. By staining ultrathin sections with anti-CA IV/immunogold, CA IV could also be demonstrated at the latter two locations, including the specialized sarcolemmal structures intercalated discs, and T-tubules. In addition, by this method CA IV was seen to be associated with the sarcoplasmic reticulum (SR). The absence of immunostaining in SR and/or SL with some techniques probably indicates a problem of accessibility of the antigenic sites. In line with the immunohistochemical results, CA IV mRNA expression was visualized in both endothelial and muscle cells by in situ hybridization histochemistry.
我们采用免疫组织化学方法，通过光学显微镜和电子显微镜研究了大鼠和人类心脏中的碳酸酐酶IV（CA IV）。在用抗CA IV/FITC孵育的冰冻切片中，毛细血管对CA IV呈现强烈反应。在用抗CA IV/ABC（抗生物素蛋白-生物素-过氧化物酶复合物）处理的石蜡切片和半薄切片中，血管、毛细血管和肌膜（SL）呈阳性染色。通过用抗CA IV/免疫金对超薄切片进行染色，也能在上述后两个位置显示出CA IV，包括闰盘和T小管等特化的肌膜结构。此外，通过这种方法可以看到CA IV与肌浆网（SR）相关。某些技术在SR和/或SL中未出现免疫染色，这可能表明抗原位点的可及性存在问题。与免疫组织化学结果一致，通过原位杂交组织化学在内皮细胞和肌肉细胞中均观察到了CA IV mRNA的表达。
