Krylov M Iu, Rokhlin O V
Mol Biol (Mosk). 1978 May-Jun;12(3):511-7.
The role of light kappa and lambda chains and also allelic variants of kappa chains of rat immunoglobins in the formation of antibodies to beta-N-acetyl-glucosamine polysaccharides of streptococcus group A of inbred rat strains MSU, WAG, August and hybrids of the first generation (MUS X WAG)F1 and (MSU X August)F1 was studied. From individual sera of immune rats fractions of specific antibodies to beta-N-acetyl-glucosamine were isolated. These antibodies differ in their affinity to antigenes. The retio of molecules with kappa and lambda light chain types was determined for the fraction of specific antibodies. The ratio of molecules kappa and lambda depends on the affinity of antibodies to beta-N-acetyl-glucosamine and on the genotype of the animals studied. Data obtained allow to conclude that differences in the functional activity of lambda chains between strains WAG and August, on one hand, and strain MSU, on the other, do exist. Functional differences releaved between these rat strains were confirmed by analyzing corresponding antibody fractions to beta-N-acetylglucosamine in F1 hybrids. Differences between allelic variants of kappa chains in the formation of antibodies to beta-N-acetylglucosamine of polysaccharides were not found.
研究了大鼠免疫球蛋白的轻链κ和λ以及κ链的等位基因变体在近交系大鼠MSU、WAG、奥古斯特以及第一代杂种（MUS×WAG）F1和（MSU×奥古斯特）F1针对A组链球菌β-N-乙酰葡糖胺多糖形成抗体过程中的作用。从免疫大鼠的个体血清中分离出针对β-N-乙酰葡糖胺的特异性抗体组分。这些抗体对抗原的亲和力不同。测定了特异性抗体组分中κ和λ轻链类型分子的比例。κ和λ分子的比例取决于抗体对β-N-乙酰葡糖胺的亲和力以及所研究动物的基因型。所得数据表明，一方面WAG和奥古斯特品系与另一方面MSU品系之间，λ链的功能活性确实存在差异。通过分析F1杂种中针对β-N-乙酰葡糖胺的相应抗体组分，证实了这些大鼠品系之间存在的功能差异。未发现κ链等位基因变体在多糖β-N-乙酰葡糖胺抗体形成过程中的差异。
