[IgM-IgG抗体合成的转换。I.不同类别的抗乳糖苷抗体中轻链大鼠免疫球蛋白链等位基因变体的比例]

[Switch in the synthesis of IgM--IgG antibodies. I. Ratio of allelic variants of light rat immunoglobulin chains in antilactoside antibodies of different classes].

作者信息

Rokhlin O V

出版信息

Mol Biol (Mosk). 1976 Jan-Feb;10(1):206-15.

Abstract

Ratio of allelic variants was studied of the rat immunoglobulin light chains in IgM and IgG fractions of anti-lactoside antibodies isolated from the sera of immune heterozygous rats (WAGXMSU)F1 and (AugustXMSU)F1. For MSU rat strain, RL1 allelic variant of light chains is typical whereas WAG and August strains possess the RL2 variant. Anti-lactoside antibodies isolated from sera of F1 rats were separated into high affinity and low affinity fractions. Thereafter each fraction was divided into IgG and IgM classes and each preparation was tested for the content of the molecules of the antibodies with light chains of RL1 and RL2 type. It has been shown that the ratio of allelic variants coincides in IgM and IgG antibody fractions. It follows from this fact that the IgG switch of antibody synthesis may process in the same cell during development of the given population of antibody-producing cells.

摘要

研究了从免疫杂合大鼠（WAGXMSU）F1和（AugustXMSU）F1血清中分离出的抗乳糖苷抗体的IgM和IgG组分中大鼠免疫球蛋白轻链的等位基因变体比例。对于MSU大鼠品系，轻链的RL1等位基因变体是典型的，而WAG和August品系则具有RL2变体。从F1大鼠血清中分离出的抗乳糖苷抗体被分为高亲和力和低亲和力组分。此后，每个组分又被分为IgG和IgM类别，并对每种制剂中具有RL1和RL2型轻链的抗体分子含量进行检测。结果表明，等位基因变体在IgM和IgG抗体组分中的比例是一致的。由此事实可知，在给定的抗体产生细胞群体发育过程中，抗体合成的IgG转换可能在同一细胞中进行。