转基因大肠杆菌生产D-乙偶姻。
The production of D-acetoin by a transgenic Escherichia coli.
作者信息
Ui S, Mimura A, Okuma M, Kudo T
机构信息
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Japan.
出版信息
Lett Appl Microbiol. 1998 Apr;26(4):275-8. doi: 10.1046/j.1472-765x.1998.00313.x.
A 6 kbp SphI fragment encoding genes for the enzymes alpha-acetolactate synthase (ALS), alpha-acetolactate decarboxylase (ALDC) and meso-2,3-butanediol dehydrogenase (meso-BDH), involved in the formation of meso-2,3-butanediol (meso-BD) from pyruvic acid, was cloned into plasmid pUC118. When derivatives of this plasmid were introduced into Escherichia coli JM109, the transformants were able to produce D-acetoin (D-AC) without contamination with L-acetoin (L-AC).
一个6千碱基对的SphI片段，编码参与从丙酮酸形成内消旋-2,3-丁二醇（meso-BD）的α-乙酰乳酸合酶（ALS）、α-乙酰乳酸脱羧酶（ALDC）和内消旋-2,3-丁二醇脱氢酶（meso-BDH）的基因，被克隆到质粒pUC118中。当将该质粒的衍生物导入大肠杆菌JM109时，转化体能够产生D-乙偶姻（D-AC）而不被L-乙偶姻（L-AC）污染。
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