Saenko A S, Zamulaeva I A, Smirnova S G, Orlova N V, Selivanova E I, Nugis V Iu, Nadezhina N M
Medical Radiological Research Center, Russian Academy Medical Sciences, Obninsk.
Radiats Biol Radioecol. 1998 Mar-Apr;38(2):171-80.
The frequencies of somatic mutations at loci of glycophorin A (GPA) and T-cell receptor (TCR) were determined in persons exposed professionally to ionizing radiation or a result of accidents at nuclear power plants and in control donors. Dependence of glycophorin A mutant (NO) cell frequency on doses of acute (up to 3.5 Gy) and prolonged (up to 15.0 Gy) irradiation was shown. The slope of linear regression corresponded to increase of NO-mutant frequency by 31.1 x 10(-6)/Gy (r = 0.87, p < 0.0001) for acute irradiation and by 6.3 x 10(-6)/Gy (r = 0.61, p < 0.0001) for prolonged one. The 5-fold decrease of the linear regression slope in the case of prolonged irradiation makes significantly worse permissive ability of the GPA test. Therefore its use for biological dosimetry of prolonged irradiation is not expedient in dose interval up to 1 Gy. The frequency of mutations in genes of T-cell receptor significantly correlated with dose of irradiation only in group of donors with recent radiation exposure (r = 0.75, p = 0.0002). Meanwhile, the TCR method is more sensitive and informative for biological dosimetry of recent radiation, than the GPA test.
测定了职业性暴露于电离辐射或因核电站事故而受辐射的人员以及对照供者中糖蛋白A（GPA）和T细胞受体（TCR）基因座的体细胞突变频率。结果显示了糖蛋白A突变（NO）细胞频率与急性照射（最高3.5 Gy）和长期照射（最高15.0 Gy）剂量的相关性。线性回归斜率显示，急性照射时NO突变频率每Gy增加31.1×10⁻⁶（r = 0.87，p < 0.0001），长期照射时每Gy增加6.3×10⁻⁶（r = 0.61，p < 0.0001）。长期照射时线性回归斜率下降5倍，使得GPA试验的许可能力显著变差。因此，在剂量区间达1 Gy时，将其用于长期照射的生物剂量测定并不合适。仅在近期有辐射暴露的供者组中，T细胞受体基因突变频率与照射剂量显著相关（r = 0.75，p = 0.0002）。同时，对于近期辐射的生物剂量测定，TCR方法比GPA试验更敏感且信息更丰富。
