Song H, Seishima M, Saito K, Maeda S, Takemura M, Noma A, Kondo A, Manabe M, Urakami K, Nakashima K
Department of Laboratory Medicine, Gifu University School of Medicine, Japan.
Ann Clin Biochem. 1998 May;35 ( Pt 3):408-14. doi: 10.1177/000456329803500310.
It has been demonstrated that apolipoproteins found in cerebrospinal fluid (CSF) play an important role in lipid metabolism in the central nervous system (CNS). Previously we reported that CSF apo A-I levels increased with the severity of neurological damage in poliovirus-infected macaques. In the present study, apo A-I was quantitatively analysed in CSF from patients with or without neurological diseases. In controls, CSF apo A-I level was significantly higher in males; 3.83 (0.40) mg/L, mean (SEM) (n = 19) compared with females, 2.42 (0.26) mg/L (n = 23, P < 0.05). CSF apo A-I concentrations in patients with acute meningitis increased at the active stage, 7.74 (1.78) mg/L (n = 10), but returned to basal concentrations at the convalescent stage 2.72 (0.38) mg/L (n = 10), while the CSF apo A-I level in patients with other neurological diseases remained in the same range as in controls. By contrast, CSF apo E was consistently elevated at either stage of acute meningitis. Furthermore, it was found that the levels of CSF apo A-I, but not of apo E, correlated positively with CSF albumin concentrations. These findings suggest that the CSF apo A-I and apo E have different origins and may play different roles in the lipoprotein metabolism in CNS.
已证明，脑脊液（CSF）中发现的载脂蛋白在中枢神经系统（CNS）的脂质代谢中起重要作用。此前我们报道，在感染脊髓灰质炎病毒的猕猴中，脑脊液载脂蛋白A-I水平随神经损伤的严重程度增加而升高。在本研究中，对患有或未患有神经疾病的患者的脑脊液中的载脂蛋白A-I进行了定量分析。在对照组中，男性脑脊液载脂蛋白A-I水平显著更高；平均（标准误）为3.83（0.40）mg/L（n = 19），而女性为2.42（0.26）mg/L（n = 23，P < 0.05）。急性脑膜炎患者在活动期脑脊液载脂蛋白A-I浓度升高，为7.74（1.78）mg/L（n = 10），但在恢复期恢复到基础浓度，为2.72（0.38）mg/L（n = 10），而其他神经疾病患者的脑脊液载脂蛋白A-I水平与对照组保持在相同范围内。相比之下，急性脑膜炎任一阶段脑脊液载脂蛋白E均持续升高。此外，发现脑脊液载脂蛋白A-I水平而非载脂蛋白E水平与脑脊液白蛋白浓度呈正相关。这些发现表明，脑脊液载脂蛋白A-I和载脂蛋白E有不同的来源，可能在中枢神经系统的脂蛋白代谢中发挥不同作用。
