Lapshina E A, Jaruga E, Biliński T, Bartosz G
Department of Molecular Biophysics, University of Lódź, Poland.
Biochem Mol Biol Int. 1995 Nov;37(5):903-8.
Reactivity which organic radicals was compared in yeast (Saccharomyces cerevisiae) strains defective in catalase and superoxide dismutase, and with a decreased level of glutathione. Yeast cell homogenates did not show considerable strain-related differences in the ability to scavenge nitroxide (TEMPO) stable free radicals and alkoxyl free radicals generated by decomposition of the free radical initiator AAPH. The "total antioxidant status" based on scavenging of ABTS free radicals showed a good correlation with the radiation resistance of the yeasts. These results point to the importance of other factors, apart from antioxidative enzymes and glutathione, in the determination of cellular resistance to ionizing radiation and other types of free-radical stress.
对过氧化氢酶和超氧化物歧化酶缺陷且谷胱甘肽水平降低的酵母（酿酒酵母）菌株中有机自由基的反应性进行了比较。酵母细胞匀浆在清除氮氧化物（TEMPO）稳定自由基和自由基引发剂AAPH分解产生的烷氧基自由基的能力上，未表现出明显的菌株相关差异。基于ABTS自由基清除的“总抗氧化状态”与酵母的抗辐射性具有良好的相关性。这些结果表明，除了抗氧化酶和谷胱甘肽外，其他因素在决定细胞对电离辐射和其他类型自由基应激的抗性方面也很重要。
