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外部引导序列和核糖核酸酶P对培养细胞中流感病毒产生的有效抑制

Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P.

作者信息

Plehn-Dujowich D, Altman S

机构信息

Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.

出版信息

Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7327-32. doi: 10.1073/pnas.95.13.7327.

DOI:10.1073/pnas.95.13.7327
PMID:9636148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC22606/
Abstract

The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.

摘要

流感病毒基因组编码的聚合酶（PB2）基因和核衣壳（NP）基因对于病毒复制至关重要。当在组织培养中，将表达针对PB2基因和NP基因mRNA的外部引导序列RNA的合成基因稳定整合到小鼠细胞中时，用流感病毒感染这些细胞不会产生子代病毒。当靶向的流感病毒mRNA与外部引导序列形成复合物时，内源性核糖核酸酶P会切割这些mRNA。同时靶向两种不同的mRNA比仅靶向一种mRNA能更有效地抑制病毒颗粒的产生。