Wells J M, Cutler H G, Cole R J
Can J Microbiol. 1976 Aug;22(8):1137-43. doi: 10.1139/m76-165.
A biologically active metabolite was found in crude extracts of an unidentified species of Phomopsis isolated from weevil-damaged pecans. Purified metabolite was toxic to day-old cockerels, with an LD50 of 12.5 mg/kg, and was markedly inhibitory to growth the floral development of tobacco plants at concentrations of 10(-2) to 10(-4) M. IR and 1H-nmr spectra indicated an alkylpheny moiety with a single nitrogen atom present as a secondary amide; and acetate ester, OH, CH3, and a trans-double bond function. Elemental and high-resolution mass-spectral analysis indicated a formula of C30H39NO5 and a molecular weight of 493.2886. The metabolite was identified as cytochalasin H.
从象鼻虫侵害的山核桃中分离出一种未鉴定的拟茎点霉属物种，在其粗提物中发现了一种生物活性代谢物。纯化后的代谢物对一日龄小公鸡有毒，半数致死剂量为12.5毫克/千克，在浓度为10⁻²至10⁻⁴摩尔时，对烟草植株的生长和花芽发育有显著抑制作用。红外光谱和¹H-核磁共振光谱表明，该代谢物含有一个带有单个氮原子的烷基苯基部分，以仲酰胺形式存在；还有一个乙酸酯基、羟基、甲基和一个反式双键官能团。元素分析和高分辨率质谱分析表明其分子式为C₃₀H₃₉NO₅，分子量为493.2886。该代谢物被鉴定为细胞松弛素H。
