Seet L F, Cho S, Hessel A, Dumont D J
Ontario Cancer Institutes, Toronto, Canada.
Biochem Biophys Res Commun. 1998 Jun 9;247(1):116-22. doi: 10.1006/bbrc.1998.8564.
Synaptojanin 2 is an inositol polyphosphate 5'-phosphatase that appears to be regulated by alternative splicing. By screening mouse cDNA libraries derived from either mouse day 16 embryo or adult liver, we have identified additional synaptojanin 2 cDNAs that represent six new isoforms of the protein. This finding, together with other reports, indicates the presence of eight isoforms of synaptojanin 2. Sequence analysis of our cDNA clones suggests that there are at least two putative initiation sites and at least six different sequences coding for the carboxyl-terminus of the molecule. In addition, we have mapped synaptojanin 2 to mouse chromosome 17 band A2-3.1 by fluorescence in situ hybridization.
突触结合蛋白2是一种肌醇多磷酸5'-磷酸酶，似乎受可变剪接调控。通过筛选来自小鼠16天胚胎或成年肝脏的cDNA文库，我们鉴定出了额外的突触结合蛋白2 cDNA，它们代表该蛋白的六种新亚型。这一发现与其他报道一起，表明存在八种突触结合蛋白2亚型。对我们的cDNA克隆进行序列分析表明，该分子至少有两个推定的起始位点以及至少六个编码羧基末端的不同序列。此外，我们通过荧光原位杂交将突触结合蛋白2定位到小鼠17号染色体A2-3.1带。
