Manners D J, Stark J R, Wilson G, Brodie J Y
Carbohydr Res. 1976 Jul;49:383-8. doi: 10.1016/s0008-6215(00)83155-9.
A commercial enzyme preparation, of fungal origin, contained a mixture of beta-D-glucanases which were fractionated by ion-exchange chromatography to give a mixture of an endo-(1 leads to 4)- and an exo-(1 leads to 3)-beta-D-glucanase. These two enzymes were then separated by molecular-sieve chromatography on Sephadex G-150. The purified exo-(1 leads to 3)-beta-D-glucanase has a relatively high specificity for (1 leads to 3)-beta-D-glucosidic linkages, and has no action on lichenin.
一种源自真菌的商业酶制剂含有β-D-葡聚糖酶混合物,通过离子交换色谱法对其进行分级分离,得到一种内切-(1→4)-和一种外切-(1→3)-β-D-葡聚糖酶的混合物。然后通过在葡聚糖G-150上进行分子筛色谱法将这两种酶分离。纯化后的外切-(1→3)-β-D-葡聚糖酶对(1→3)-β-D-糖苷键具有相对较高的特异性,并且对地衣多糖无作用。