Miller A H, Spencer R L, Pearce B D, Pisell T L, Azrieli Y, Tanapat P, Moday H, Rhee R, McEwen B S
Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Cell Immunol. 1998 May 25;186(1):45-54. doi: 10.1006/cimm.1998.1293.
Cytosolic glucocorticoid receptor (GR) binding studies on immune tissues demonstrate that the thymus exhibits three to four times higher levels of GR protein than the spleen. High levels of GR are consistent with the exquisite sensitivity of the thymus to glucocorticoid exposure. Nevertheless, whole cell binding studies reveal similar levels of GR in immature thymic T lymphocytes and more mature, splenic T lymphocytes. Moreover, whole cell binding techniques indicate that neutrophils (which represent roughly 30% of splenic leukocytes) exhibit higher GR than both T and B lymphocytes, further contradicting results from cytosolic binding studies. To address these inconsistencies, GR protein was assessed in immune cells and tissues using cytosolic radioligand binding. Western blot analysis, and immunocytochemistry. Consistent with previous cytosolic receptor binding studies on immune tissue homogenates, thymic T cells were found to have higher levels of GR than T cells isolated from the spleen. In addition, neutrophils were found to have fewer GR than lymphocytes and monocytes. These results indicate a meaningful relationship between receptor expression and known sensitivity to glucocorticoids.
对免疫组织进行的胞质糖皮质激素受体（GR）结合研究表明，胸腺中GR蛋白水平比脾脏高3至4倍。高水平的GR与胸腺对糖皮质激素暴露的高度敏感性相一致。然而，全细胞结合研究显示，未成熟胸腺T淋巴细胞和更成熟的脾脏T淋巴细胞中的GR水平相似。此外，全细胞结合技术表明，中性粒细胞（约占脾脏白细胞的30%）的GR比T淋巴细胞和B淋巴细胞都高，这进一步与胞质结合研究的结果相矛盾。为了解决这些不一致的问题，使用胞质放射性配体结合、蛋白质免疫印迹分析和免疫细胞化学方法对免疫细胞和组织中的GR蛋白进行了评估。与之前对免疫组织匀浆进行的胞质受体结合研究一致，发现胸腺T细胞中的GR水平高于从脾脏分离的T细胞。此外，还发现中性粒细胞中的GR比淋巴细胞和单核细胞少。这些结果表明受体表达与已知的对糖皮质激素的敏感性之间存在有意义的关系。
