Sugiura T, Kondo T, Imagawa H, Kamada M
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi-ken, Japan.
Vet Immunol Immunopathol. 1998 Mar 31;62(2):145-51. doi: 10.1016/s0165-2427(97)00161-x.
Stable clones of 18 mouse hybridomas that produce monoclonal antibodies (MAbs) to six isotypes of horse immunoglobulin were produced. The number of hybridomas of different specificities are: four to IgGa, four to IgGb, one to IgGc, four to IgG(T), two to IgM, and three to IgA. The immunoglobulin isotypes purified from affinity columns bound with each MAb showed variable mobility in agarose-gel electrophoresis. Migration proceeded from the anode as: IgG(T), IgA, IgM, IgGc, IgGb and IgGa. The purified isotypes also demonstrated a single precipitin line when in immunoelectrophoresis against isotype-specific or polyclonal antisera, raised against the purified isotype or whole equine serum, respectively.
产生针对马免疫球蛋白六种同种型的单克隆抗体（MAb）的18个小鼠杂交瘤稳定克隆被制备出来。不同特异性杂交瘤的数量分别为：针对IgGa的有4个，针对IgGb的有4个，针对IgGc的有1个，针对IgG(T)的有4个，针对IgM的有2个，针对IgA的有3个。从与每种单克隆抗体结合的亲和柱上纯化得到的免疫球蛋白同种型在琼脂糖凝胶电泳中显示出可变的迁移率。迁移从阳极开始依次为：IgG(T)、IgA、IgM、IgGc、IgGb和IgGa。当针对分别用纯化的同种型或全马血清制备的同种型特异性或多克隆抗血清进行免疫电泳时，纯化的同种型也显示出单一沉淀线。
