Figeys D, Aebersold R
Department of Molecular Biotechnology, University of Washington, Seattle, USA.
Electrophoresis. 1998 May;19(6):885-92. doi: 10.1002/elps.1150190603.
Analytical biochemistry, in particular the analysis of regulatory proteins that control biological systems and pathways, is dependent on methods of ever-increasing sensitivity. Capillary electrophoresis (CE) has long been recognized as an ultrasensitive analytical technique. In spite of the high sensitivity, CE has not penetrated protein discovery research as a standard analytical method. In this review article we summarize recent technical developments which have significantly enhanced CE as a tool for the analysis of trace amounts of proteins. Specifically, we review recent advances in the development and application of capillary electrophoresis-mass spectrometry (CE-MS) and on-line analyte concentration techniques, and introduce the emerging field of microfluidics as a front end to mass spectrometry (MS).
分析生物化学，尤其是对控制生物系统和途径的调节蛋白的分析，依赖于灵敏度不断提高的方法。毛细管电泳（CE）长期以来一直被认为是一种超灵敏的分析技术。尽管灵敏度很高，但CE尚未作为一种标准分析方法渗透到蛋白质发现研究中。在这篇综述文章中，我们总结了最近的技术发展，这些发展显著增强了CE作为分析痕量蛋白质的工具的能力。具体来说，我们回顾了毛细管电泳-质谱联用（CE-MS）和在线分析物浓缩技术的发展与应用方面的最新进展，并介绍了作为质谱（MS）前端的微流控新兴领域。
