Okosi A, Brar B K, Chan M, D'Souza L, Smith E, Stephanou A, Latchman D S, Chowdrey H S, Knight R A
Department of Cystic Fibrosis, National Heart and Lung Institute, Imperial College, London, UK.
Neuropeptides. 1998 Apr;32(2):167-71. doi: 10.1016/s0143-4179(98)90033-6.
Reverse transcription PCR showed that mRNA encoding the CRH-like molecule, urocortin, is expressed in a rat cardiac myocyte cell line and in primary cultures of cardiac myocytes. Identity of the amplified with the published sequence was established by restriction mapping and direct sequencing. Expression of urocortin mRNA was increased 12-18 h after thermal injury. Urocortin peptide protected cardiac myocytes from cell death induced by hypoxia. The data suggest that urocortin is an endogenous cardiac myocyte peptide which modulates the cellular response to stress.
逆转录聚合酶链反应表明，编码促肾上腺皮质激素释放激素样分子——尿皮质素的信使核糖核酸在大鼠心肌细胞系及原代培养的心肌细胞中表达。通过限制性酶切图谱分析和直接测序确定了扩增产物与已发表序列的一致性。热损伤后12 - 18小时，尿皮质素信使核糖核酸的表达增加。尿皮质素肽可保护心肌细胞免受缺氧诱导的细胞死亡。这些数据表明，尿皮质素是一种内源性心肌细胞肽，可调节细胞对应激的反应。
