Kobayashi T, Kubo E, Takahashi Y, Kasahara T, Yonezawa H, Akagi Y
Department of Ophthalmology, Fukui Medical University, Japan.
Arch Ophthalmol. 1998 Jun;116(6):785-9. doi: 10.1001/archopht.116.6.785.
Retinal lesions similar to those in human early-stage diabetic retinopathy have been reported to occur in dogs fed galactose for long periods. Investigations of retinal changes, however, have been limited to studies of the intact retinal vasculature isolated by trypsin digestion.
To document the onset and progression of retinal lesions in galactose-fed dogs by the common clinical techniques of fundus color photography and fluorescein angiography.
Fourteen 6-month-old male beagles made aphakic in 1 eye were divided into a control group (4 dogs), receiving a diet containing 30% cellulose, and a galactosemic group (10 dogs), receiving a diet containing 30% galactose. The progression of retinal changes in these dogs was periodically monitored by color fundus photography and fluorescein angiography.
Dogs fed a 30% galactose diet for 28 to 41 months were observed by fluorescein angiography and color fundus photography to develop, in order of frequency, microaneurysms, retinal hemorrhages, intraretinal microvascular abnormalities, retinal nonperfused areas, and varicose and serpiginous veins. These findings are similar to the early clinical retinal changes observed in humans with diabetes.
These results confirm that galactosemic dogs are an appropriate and suitable animal model for investigating human diabetic retinopathy.
据报道，长期喂食半乳糖的犬类会出现与人类早期糖尿病视网膜病变相似的视网膜病变。然而，对视网膜变化的研究仅限于通过胰蛋白酶消化分离的完整视网膜血管系统的研究。
通过眼底彩色摄影和荧光素血管造影等常见临床技术，记录喂食半乳糖的犬类视网膜病变的发生和发展过程。
将14只6个月大的雄性比格犬的一只眼睛制成无晶状体眼，分为对照组（4只犬），给予含30%纤维素的饮食，以及半乳糖血症组（10只犬），给予含30%半乳糖的饮食。通过彩色眼底摄影和荧光素血管造影定期监测这些犬类视网膜变化的进展。
通过荧光素血管造影和彩色眼底摄影观察到，喂食30%半乳糖饮食28至41个月的犬类，按出现频率依次出现微动脉瘤、视网膜出血、视网膜内微血管异常、视网膜无灌注区以及静脉曲张和迂曲静脉。这些发现与糖尿病患者早期临床视网膜变化相似。
这些结果证实，半乳糖血症犬是研究人类糖尿病视网膜病变的合适动物模型。
