Solovei I V, Joffe B I, Hori T, Thomson P, Mizuno S, Macgregor H C
Institute of Anthropology and Human Genetics, University of Munich, Munich,
Chromosoma. 1998 Jun;107(3):184-8. doi: 10.1007/s004120050295.
The arrangement of chromosomes in the elongated sperm nuclei of chicken was studied using fluorescence in situ hybridization with probes specific for telomeres of all chromosomes, a microchromosome, the long arm of chromosome 6, the large heterochromatic block on the Z-chromosome, and the same heterochromatic block plus subtelomeric sites on macrochromosomes 1-4. The positions of all probes vary from one sperm to another. No order in chromosome arrangement is apparent. It is suggested that large chromosome size and small chromosome number correlate with constant positions of chromosomes and vice versa. Based on the known quantity of repetitive units of the repeat on the Z-chromosome, the degree of compaction of chromatin in the chicken sperm nucleus is estimated as ca 0.7 Mb/ microm. As judged from the length of the heterochromatic region of the Z-chromosome at the lampbrush stage, the total length of the Z-chromosome in mature sperm is 2.5-4 times that of the sperm nucleus.
利用荧光原位杂交技术，使用针对所有染色体端粒、一条微染色体、6号染色体长臂、Z染色体上的大异染色质块以及1 - 4号常染色体上相同异染色质块加亚端粒位点的探针，研究了鸡细长精子核中染色体的排列情况。所有探针的位置在不同精子之间各不相同。染色体排列没有明显顺序。有人提出，染色体体积大、数量少与染色体位置固定相关，反之亦然。根据Z染色体上已知的重复序列重复单元数量，估计鸡精子核中染色质的压缩程度约为0.7 Mb/μm。从灯刷期Z染色体异染色质区域的长度判断，成熟精子中Z染色体的总长度是精子核长度的2.5 - 4倍。
