Krishnamurthy H, Jagetia G C, Jyothi P
Department of Radiobiology, Kasturba Medical College, Manipal 576 119, India.
Mutat Res. 1998 Jun 5;401(1-2):111-20. doi: 10.1016/s0027-5107(97)00320-5.
The radioprotective effect of zinc aspartate on spermatogonial cells of whole-body irradiated mice was studied using flow cytometry. Adult male Swiss albino mice were treated with 30 mg/kg body weight of zinc aspartate 30 min before exposure to 0, 0.5, 1, 2 and 3 Gy of gamma-radiation. The animals were killed 7 to 70 days postirradiation and the relative percentages of different germ cells were analyzed by flow cytometry. A significant increase (p<0.002, 0. 0001, 0.005 and 0.008 for 0.5, 1, 2 and 3 Gy, respectively) in the relative percentage of spermatogonial (2C) population was observed in mice treated with zinc aspartate before exposure to different doses of gamma-radiation, compared to the irradiated controls on day 35 posttreatment. Also mean of each radiation dose of all the intervals studied showed a significant (p<0.03) increase in the relative percentage of spermatogonia. Despite the increase in the relative percentage of spermatogonia, the relative percentage of tetraploid cells (4C) remained higher in the zinc aspartate treated mice, compared to the irradiated controls. However, there was no change in the haploid populations viz. round (1C) and elongated (HC) spermatids of the zinc aspartate pretreated animals compared to irradiated controls. These data suggests that zinc aspartate pretreatment protects spermatogonia and tetraploid cells from radiation-induced cell killing.
采用流式细胞术研究了天冬氨酸锌对全身照射小鼠精原细胞的辐射防护作用。成年雄性瑞士白化小鼠在接受0、0.5、1、2和3 Gy的γ射线照射前30分钟，用30 mg/kg体重的天冬氨酸锌进行处理。在照射后7至70天处死动物，通过流式细胞术分析不同生殖细胞的相对百分比。与处理后第35天的照射对照组相比，在接受不同剂量γ射线照射前用天冬氨酸锌处理的小鼠中，精原细胞（2C）群体的相对百分比显著增加（分别在0.5、1、2和3 Gy时p<0.002、0.0001、0.005和0.008）。在所研究的所有时间间隔内，每个辐射剂量的平均值也显示精原细胞的相对百分比显著增加（p<0.03）。尽管精原细胞的相对百分比有所增加，但与照射对照组相比，天冬氨酸锌处理的小鼠中四倍体细胞（4C）的相对百分比仍然较高。然而，与照射对照组相比，天冬氨酸锌预处理动物的单倍体群体，即圆形（1C）和延长型（HC）精子细胞没有变化。这些数据表明，天冬氨酸锌预处理可保护精原细胞和四倍体细胞免受辐射诱导的细胞杀伤。
