Hacker U, Schumann J, Göhde W
Acta Radiol Oncol. 1980;19(5):361-8. doi: 10.3109/02841868009131321.
Mice irradiated with doses ranging from 0.1 to 15 Gy using a 60Co-source and controls were killed at intervals varying from 2 to 35 days after irradiation. The DNA content of the testicular cells in single cell suspensions was measured with the pulse cytophotometer to determine the frequencies of the different stages in the spermatogenesis. The relative amount of S-phase and 4c-cells was reduced initially but increased subsequently to hypernormal values. A decrease of 2c-cells indicated a higher cell-kill of diploid spermatogonia. Gamma ray-induced spermatids with abnormal DNA-values (diploid sperm) were identified. The results suggest that the spermatogenesis can be analysed with flow cytometry and used as a biologic dosimeter even for small doses of ionizing radiation.
使用钴 - 60源对小鼠进行0.1至15戈瑞剂量的照射，并设置对照组，在照射后2至35天的不同时间间隔处死小鼠。用脉冲细胞光度计测量单细胞悬液中睾丸细胞的DNA含量，以确定精子发生不同阶段的频率。S期和4c细胞的相对量最初减少，但随后增加至超正常水平。2c细胞的减少表明二倍体精原细胞的细胞杀伤率更高。鉴定出了γ射线诱导的具有异常DNA值的精子细胞（二倍体精子）。结果表明，精子发生可以通过流式细胞术进行分析，并用作生物剂量计，即使对于小剂量的电离辐射也是如此。
