Guzmán-Silva M A
Department of Pathology, Fluminense Federal University, Niteroi, RJ, Brazil.
Acta Cient Venez. 1997;48(3):130-3.
A simple protocol for the growth and differentiation of adult Mongolian gerbil epidermal cells is reported. Insulin (8 micrograms/ml) and reduced levels of serum supplementation (2%) were sufficient for the maintenance of these cells in culture. Primary cultures were maintained as a proliferative monolayer in a medium with low calcium concentration (< 0.3 mM). Terminal differentiation of cultures was induced by raising the calcium concentration (1.6 mM) in the medium. These results support the concept derived from mouse epidermal cell culture that calcium is an important regulator of mammalian epidermal cell growth and differentiation. The present protocol also represents a useful tool for studies of mechanisms involved in epidermal cell growth and differentiation in a laboratory animal.
报道了一种用于成年蒙古沙鼠表皮细胞生长和分化的简单方案。胰岛素（8微克/毫升）和降低的血清补充水平（2%）足以在培养中维持这些细胞。原代培养物在低钙浓度（<0.3毫摩尔/升）的培养基中作为增殖单层维持。通过提高培养基中的钙浓度（1.6毫摩尔/升）诱导培养物的终末分化。这些结果支持了从小鼠表皮细胞培养得出的概念，即钙是哺乳动物表皮细胞生长和分化的重要调节因子。本方案也是研究实验动物表皮细胞生长和分化相关机制的有用工具。
